Euler André, Blüthgen Christian, Wurnig Moritz C, Jungraithmayr Wolfgang, Boss Andreas
Institute of Diagnostic and Interventional Radiology, University Hospital Zurich, Zurich, Switzerland.
Department of Thoracic Surgery, University Hospital Rostock, Rostock, Germany.
J Magn Reson Imaging. 2020 Jan;51(1):108-116. doi: 10.1002/jmri.26817. Epub 2019 May 31.
Differentiation of early postoperative complications affects treatment options after lung transplantation.
To assess if texture analysis in ultrashort echo-time (UTE) MRI allows distinction of primary graft dysfunction (PGD) from acute transplant rejection (ATR) in a mouse lung transplant model.
Longitudinal.
Single left lung transplantation was performed in two cohorts of six mice (strain C57BL/6) receiving six syngeneic (strain C57BL/6) and six allogeneic lung transplants (strain BALB/c (H-2K )).
FIELD STRENGTH/SEQUENCE: 4.7T small-animal MRI/eight different UTE sequences (echo times: 50-5000 μs) at three different postoperative timepoints (1, 3, and 7 days after transplantation).
Nineteen different first- and higher-order texture features were computed on multiple axial slices for each combination of UTE and timepoint (24 setups) in each mouse. Texture features were compared for transplanted (graft) and contralateral native lungs between and within syngeneic and allogeneic cohorts. Histopathology served as a reference.
Nonparametric tests and correlation matrix analysis were used.
Pathology revealed PGD in the syngeneic and ATR in the allogeneic cohort. Skewness and low-gray-level run-length features were significantly different between PGD and ATR for all investigated setups (P < 0.03). These features were significantly different between graft and native lung in ATR for most setups (minimum of 20/24 setups; all P < 0.05). The number of significantly different features between PGD and ATR increased with elapsing postoperative time. Differences in significant features were highest for an echo-time of 1500 μs.
Our findings suggest that texture analysis in UTE-MRI might be a tool for the differentiation of PGD and ATR in the early postoperative phase after lung transplantation.
1 Technical Efficacy: Stage 3 J. Magn. Reson. Imaging 2020;51:108-116.
肺移植术后早期并发症的鉴别会影响治疗方案的选择。
评估超短回波时间（UTE）MRI中的纹理分析是否能够在小鼠肺移植模型中区分原发性移植功能障碍（PGD）和急性移植排斥反应（ATR）。
纵向研究。
在两组各6只小鼠（C57BL/6品系）中进行单左肺移植，一组接受6次同基因（C57BL/6品系）肺移植，另一组接受6次异基因肺移植（BALB/c（H-2K）品系）。
场强/序列：4.7T小动物MRI/在术后三个不同时间点（移植后1、3和7天）采用8种不同的UTE序列（回波时间：50 - 5000μs）。
针对每只小鼠UTE和时间点的每种组合（24种设置），在多个轴向切片上计算19种不同的一阶和高阶纹理特征。比较同基因和异基因队列中移植（移植物）肺和对侧正常肺之间以及内部的纹理特征。组织病理学作为参考。
采用非参数检验和相关矩阵分析。
病理学检查显示同基因队列中有PGD，异基因队列中有ATR。在所有研究设置中，PGD和ATR之间的偏度和低灰度游程特征存在显著差异（P < 0.03）。在大多数设置下（至少20/24种设置），ATR中移植物肺和正常肺之间的这些特征存在显著差异（所有P < 0.05）。PGD和ATR之间显著不同特征的数量随术后时间的推移而增加。回波时间为1500μs时，显著特征的差异最大。
我们的研究结果表明，UTE-MRI中的纹理分析可能是肺移植术后早期区分PGD和ATR的一种工具。
1 技术效能：3期 J. Magn. Reson. Imaging 2020;51:108 - 116。
