Key Laboratory of Agro-Food Processing and Quality Control, Institute of Food Science and Technology, Chinese Academy of Agricultural Science, Beijing, China.
R&D Center, Heilongjiang Feihe Dairy Co., Ltd, Beijing, China.
Carbohydr Polym. 2019 Sep 1;219:328-333. doi: 10.1016/j.carbpol.2019.04.092. Epub 2019 May 1.
Human milk oligosaccharides (HMOs) are beneficial to infants' growth. Currently, the challenges were the complex operation and weak separation which limited the accurate quantification of HMOs. Liquid chromatography-mass spectrometry (LC-MS/MS) under multi-reaction monitoring (MRM) mode was developed here to separate and quantitatively determine 12 oligosaccharides in human milk. The degree of polymerization of 12 oligosaccharides ranged from 3 to 6. Among 12 HMOS, four pairs of isomeric oligosaccharides (LNFPⅠand LNFPⅢ; LSTa、LSTb and LSTc; 2FL and 3FL; 3SL and 6SL) were successfully separated. Variations of HMOs among 61 individuals from Beijing, China were observed using the present method. Pairwise correlations analysis showed high correlation in abundance between LNDFH Ⅱand 6SL, LSTc and 6SL, LNDFH Ⅱ and LSTa, LSTa and LSTc, LSTa and 6SL. This method allows accurate and reliable quantitative determination of 12 oligosaccharides in human milk, and could be adopted in determining variations in HMOs among samples.
人乳寡糖（HMOs）有益于婴儿的生长。目前的挑战是操作复杂且分离能力弱，这限制了 HMOs 的准确定量。本研究建立了液相色谱-串联质谱（LC-MS/MS）在多反应监测（MRM）模式下，用于分离和定量测定人乳中的 12 种寡糖。12 种寡糖的聚合度范围为 3 至 6。在 12 种 HMOs 中，成功分离了 4 对同分异构寡糖（LNFPⅠ和 LNFPⅢ；LSTa、LSTb 和 LSTc；2FL 和 3FL；3SL 和 6SL）。本方法观察了来自中国北京的 61 个人的 HMOs 变化。成对相关分析显示 LNDFHⅡ和 6SL、LSTc 和 6SL、LNDFHⅡ和 LSTa、LSTa 和 LSTc、LSTa 和 6SL 之间的丰度存在高度相关性。该方法可用于准确可靠地定量测定人乳中的 12 种寡糖，也可用于测定样品中 HMOs 的变化。
