Research Institute of Bird's Nest, Xiamen Yan Palace Seelong Food Co., Ltd., Xiangming Road 3, Xiamen 361100, China.
Molecules. 2023 Feb 10;28(4):1703. doi: 10.3390/molecules28041703.
Sialyllactose is an acidic oligosaccharide that has an immune-protective effect against pathogens and contributes to developing the immune system and intestinal microbes. In this study, a method for the determination of 3'-sialyllactose by high-performance liquid chromatography tandem mass spectrometry was established. The sample was treated with 0.1% formic acid methanol solution, and the gradient elution was performed with 0.05% formic acid water and 0.1% formic acid acetonitrile. The hydrophilic liquid chromatographic column was used for separation. The results showed that the linearity was good in the concentration range of 1160 μg/L. The limit of detection (LOD) and the limit of quantification (LOQ) of the method were 0.3 μg/kg and 1.0 μg/kg, the recovery range was 91.6%98.4%, and the relative standard deviation (RSD) was 1.5%~2.2%. This method is fast and sensitive. In addition, the 3'-sialyllactose content in edible bird's nest products produced by different processes was studied. It was found that within the tested range, 3'-sialyllactose in edible bird's nest products increased with the intensity of stewing and increased with the addition of sugar. In short, the results provided a new method for detecting the nutritional value of edible bird's nests, as well as a new direction for improving the nutritional value of edible bird's nest products.
唾液酸乳糖是一种酸性低聚糖，具有抗病原体的免疫保护作用，并有助于免疫系统和肠道微生物的发育。本研究建立了一种高效液相色谱-串联质谱法测定 3'-唾液酸乳糖的方法。样品用 0.1%甲酸甲醇溶液处理，采用 0.05%甲酸水和 0.1%甲酸乙腈进行梯度洗脱。采用亲水液相色谱柱进行分离。结果表明，在 1160μg/L 的浓度范围内线性良好。该方法的检出限（LOD）和定量限（LOQ）分别为 0.3μg/kg 和 1.0μg/kg，回收率范围为 91.6%98.4%，相对标准偏差（RSD）为 1.5%~2.2%。该方法快速灵敏。此外，还研究了不同工艺生产的燕窝制品中 3'-唾液酸乳糖的含量。结果表明，在所测范围内，燕窝制品中的 3'-唾液酸乳糖随炖煮强度的增加而增加，随糖的加入而增加。总之，研究结果为燕窝营养价值的检测提供了一种新方法，也为提高燕窝制品的营养价值提供了一个新方向。
