Persea Naturals, LLC, State College, PA 16803, United States.
Department of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742, United States.
Food Chem. 2019 Sep 30;293:15-22. doi: 10.1016/j.foodchem.2019.04.064. Epub 2019 Apr 19.
High consumer demand has led global food color manufacturers and food companies to dramatically increase the development and use of natural colors. We have previously reported that avocado (Persea americana) seeds, when crushed in the presence of air, develop a red-orange color in a polyphenol oxidase-dependent reaction. The objective of this study was to identify the major colored compound in colored avocado seed extract (CASE). Column chromatography and high performance liquid chromatography were used to isolate the most abundant colored compound in CASE. This compound, henceforth referred to as perseorangin, was a yellow-orange solid. Structural analysis was performed using high-resolution mass spectrometry, and infrared and nuclear magnetic resonance spectroscopy. We determined that perseorangin is a glycosylated benzotropone-containing compound with a molecular formula of CHO. Liquid chromatography with electrospray ionization mass spectrometry-based metabolomic analysis of CASE and uncolored avocado seed extract showed that perseorangin was unique to CASE.
高消费者需求促使全球食品色素制造商和食品公司大力开发和使用天然色素。我们之前曾报道过，鳄梨（Persea americana）种子在空气中粉碎时，会在多酚氧化酶的作用下发生红色橙色的反应。本研究的目的是鉴定有色鳄梨籽提取物（CASE）中的主要有色化合物。通过柱层析和高效液相色谱分离 CASE 中含量最丰富的有色化合物。该化合物，此后称为 perseorangin，是一种黄橙色固体。使用高分辨率质谱、红外和核磁共振光谱进行结构分析。我们确定 perseorangin 是一种含有苯并三酮的糖基化化合物，分子式为 CHO。基于电喷雾电离质谱的 CASE 和无色鳄梨籽提取物的液相色谱代谢组学分析表明，perseorangin 是 CASE 所特有的。
