利用响应面法对真姬菇黄色素进行提取优化及纯化

Wu Jun, Zhang Jiangtao, Yu Xin, Shu Yue, Zhang Siyu, Zhang Yinglao

School of Life Science Anhui Agricultural University Hefei China.

Food Sci Nutr. 2020 Dec 4;9(2):822-832. doi: 10.1002/fsn3.2046. eCollection 2021 Feb.

var. Makiknocontains rich gardenia yellow pigment (GYP). In this study, the process of pigment extraction was optimized based on a Box-Behnken design (BBD) and response surface methodology (RSM). The absorbance and antioxidant activity (AA) were considered as responses. The result showed that the optimal extraction conditions were ethanol concentration 65.10%, liquid/solid ratio 10:1 ml/g, extraction time 59.85 min, and extraction temperature 60.04℃ for the maximal response values of absorbance (0.79) and AA (91.30%), respectively. Crude GYP was purified by the 13 different resins. The result showed that BJ-7514 was suitable for purifying GYP with the absorption ratio of 95.4%. Moreover, the 80% of ethanol eluent is applicable on the BJ-7514 with the desorption ratio of 91.93%. The major component of GYP (Crocin-3) was isolated and identified from the purified GYP.

栀子变种富含栀子黄色素（GYP）。在本研究中，基于Box-Behnken设计（BBD）和响应面方法（RSM）对色素提取工艺进行了优化。将吸光度和抗氧化活性（AA）作为响应指标。结果表明，对于吸光度（0.79）和AA（91.30%）的最大响应值，最佳提取条件分别为乙醇浓度65.10%、液固比10:1 ml/g、提取时间59.85分钟和提取温度60.04℃。采用13种不同树脂对粗GYP进行纯化。结果表明，BJ-7514适合纯化GYP，吸附率为95.4%。此外，80%的乙醇洗脱液适用于BJ-7514，解吸率为91.93%。从纯化的GYP中分离鉴定出GYP的主要成分（藏红花素-3）。