University of Birjand, Faculty of Science, Department of Chemistry, Birjand 97179-414, Iran.
University of Birjand, Faculty of Science, Department of Chemistry, Birjand 97179-414, Iran.
Food Chem. 2019 Sep 30;293:220-225. doi: 10.1016/j.foodchem.2019.04.099. Epub 2019 Apr 25.
This study was conducted for quantitative determination of quercetin in food and fruit juice samples based on a green, fast, and accurate method namely, micro-cloud point extraction (MCPE). The proposed MCPE is essentially a miniaturized form of traditional cloud point extraction (CPE) in which only a few microliters of micellar extracting phase is sufficient for determination. Factors influencing the extraction efficiency, such as amount of Triton X-114 (5%, v/v), effect of pH, amount of NaSO salt (5%, w/v) and time of centrifugation were investigated and optimized by experimental design. Under the optimized condition, the calibration range was found to be linear over 10-100 ng mL with the limit of detection of 2.2 ng mL. The relative standard deviation of 2.8%, was obtained on a 30 ng mLquercetin standard solution (n = 3). Finally, the proposed method was successfully applied for the determination of quercetin in onion, tomato, apple and orange juice samples.
本研究旨在建立一种绿色、快速、准确的定量测定食品和果汁中槲皮素的方法，即微胶束萃取（MCPE）。与传统的浊点萃取（CPE）相比，所提出的 MCPE 方法实质上是一种微型化的方法，仅需几微升的胶束萃取相即可完成测定。通过实验设计考察并优化了影响萃取效率的因素，如 Triton X-114（5%，v/v）的用量、pH 值的影响、NaSO 盐（5%，w/v）的用量和离心时间。在优化条件下，发现校准范围在 10-100ng/mL 之间呈线性关系，检出限为 2.2ng/mL。在 30ng/mL 槲皮素标准溶液（n=3）上获得了 2.8%的相对标准偏差。最后，该方法成功应用于洋葱、番茄、苹果和橙汁样品中槲皮素的测定。
