Acta Chim Slov. 2020 Dec;67(4):1092-1099.
In this study, a rapid and efficient method has been used for the extraction and determination of morin and quercetin in fruit juice samples based on air-assisted liquid-liquid microextraction based on solidification of floating organic droplet and HPLC-UV. The effects of 7 important parameters on the extraction recovery were examined and were optimized by Plackett-Burman design and Central Composite design. According to the Plackett-Burman design results, ionic strength of the sample solutions, the aspiration/dispersion cycles, and the rate and time of centrifuge did not show significant effects on the extraction of morin and quercetin. The optimized conditions of extraction were as follows; the volume of the extraction solvent of 83.6 µL, pH of 4.34 for the sample, and 1-undecanol as extraction solvent. Under these conditions, the linear calibration curve was in the ranges of 1-1000 ng/mL and 0.5-1000 ng/mL for morin and quercetin, respectively, with the determination coefficient values above 0.99. The limit of detection of morin and quercetin was 0.3 and 0.2 ng/mL, respectively. The extraction recoveries for 10 ng/mL of morin and quercetin were 98.9% and 96.5%, respectively; while, relative standard deviations (n = 3) were lower than 3.2%.
在这项研究中，使用了一种基于空气辅助液 - 液微萃取（基于固 定浮质有机液滴）和高效液相色谱-紫外检测的快速高效方法，用于从果汁样品中提取和测定杨梅素和槲皮素。考察了 7 个重要参数对萃取回收率的影响，并通过 Plackett-Burman 设计和中心复合设计进行了优化。根据 Plackett-Burman 设计结果，样品溶液的离子强度、抽吸/分散循环次数以及离心的速度和时间对杨梅素和槲皮素的萃取没有显著影响。优化的萃取条件如下：萃取溶剂体积为 83.6µL，样品 pH 值为 4.34，十一烷醇为萃取溶剂。在这些条件下，杨梅素和槲皮素的线性校准曲线范围分别为 1-1000ng/mL 和 0.5-1000ng/mL，相关系数值均大于 0.99。杨梅素和槲皮素的检测限分别为 0.3 和 0.2ng/mL。10ng/mL 杨梅素和槲皮素的萃取回收率分别为 98.9%和 96.5%，而相对标准偏差（n=3）低于 3.2%。
