School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Qinggongyuan 1, Ganjingzi District, Dalian 116034, China.
Department of Endocrinology, Dalian Municipal Central Hospital of Dalian Medical University, Dalian 116033, China.
Food Chem. 2019 Sep 30;293:387-395. doi: 10.1016/j.foodchem.2019.05.017. Epub 2019 May 3.
The endogenous carbon quantum dots (CQDs) produced during food processing have potential uncertainty to human health. The objective of this study was to investigate the formation, biodistribution and cytotoxicity of CQDs in roasted Atlantic salmon (Salmo salar Linnaeus). The X-ray photoelectron spectroscopy (XPS) analysis showed that the CQDs were mainly composed of carbon, oxygen and nitrogen. The morphology, functional groups and optical properties were highly dependent on the roasting time. In vivo experiments in mice demonstrated that the CQDs distributed in the digestive tract, kidney, liver, and even brain, which indicated that they could cross the blood-brain barrier. The cell imaging results indicated that the CQDs could readily gain access to the normal rat kidney (NRK) cells, and caused autophagosome formation. The proportion of live cells decreased to 34.62% at 6 mg mL of CQDs, and the energy generation route was changed from aerobic to glycolytic metabolism.
在食品加工过程中产生的内源性碳量子点（CQDs）对人类健康具有潜在的不确定性。本研究旨在探讨大西洋鲑（Salmo salar Linnaeus）在烤制过程中 CQDs 的形成、分布和细胞毒性。X 射线光电子能谱（XPS）分析表明，CQDs 主要由碳、氧和氮组成。形态、官能团和光学性质高度依赖于烤制时间。在小鼠体内实验中，CQDs 分布在消化道、肾脏、肝脏，甚至大脑中，这表明它们可以穿过血脑屏障。细胞成像结果表明，CQDs 可以轻易进入正常大鼠肾细胞（NRK），并引起自噬体的形成。CQDs 浓度为 6mg/mL 时，活细胞的比例下降到 34.62%，能量产生途径从有氧代谢转变为糖酵解代谢。
