直接进样-液相色谱-串联质谱法同时测定葡萄酒中的农药和真菌毒素。
Simultaneous determination of pesticides and mycotoxins in wine by direct injection and liquid chromatography-tandem mass spectrometry analysis.
机构信息
Center of Research and Analysis of Residues and Contaminants (CEPARC), Federal University of Santa Maria (UFSM), Chemistry Department, Santa Maria, RS, Brazil.
Center of Research and Analysis of Residues and Contaminants (CEPARC), Federal University of Santa Maria (UFSM), Chemistry Department, Santa Maria, RS, Brazil.
出版信息
Food Chem. 2019 Sep 30;293:83-91. doi: 10.1016/j.foodchem.2019.04.088. Epub 2019 Apr 25.
A simple and rapid method for simultaneous determination of pesticides and mycotoxins in red wine is presented. Sample preparation approach, called direct injection, consists of a sequence of only three steps: centrifugation, dilution and filtration. The analysis of extracts were performed by UPLC-MS/MS for determination of pesticides and mycotoxins. The method was assessed for linearity, limits of detection and quantification, matrix effects, selectivity, accuracy and precision. For recovery experiments, mycotoxins were divided in two groups according to their sensitivity in the UPLC-MS/MS system. More than 80% of the mycotoxins were reliably quantified at the lowest spike level studied (1 μg kg for group 1 and 50 μg kg for group 2). From the 185 evaluated pesticides, 144 showed acceptable results at 10 μg kg, the lowest spiked level. Matrix effects were, in most of the cases, negative, and that was observed for both pesticides and mycotoxins.
本研究提出了一种简单、快速测定红酒中农药和真菌毒素的方法。样品制备方法称为直接进样，仅包括三个步骤：离心、稀释和过滤。采用 UPLC-MS/MS 对提取物进行分析，以测定农药和真菌毒素。该方法的线性、检测限和定量限、基质效应、选择性、准确度和精密度均进行了评估。对于回收实验，根据真菌毒素在 UPLC-MS/MS 系统中的灵敏度，将真菌毒素分为两组。在研究的最低加标水平（第 1 组为 1μg/kg，第 2 组为 50μg/kg）下，超过 80%的真菌毒素可可靠定量。在所评估的 185 种农药中，144 种在 10μg/kg 的最低加标水平下具有可接受的结果。在大多数情况下，基质效应为负，无论是对农药还是真菌毒素都是如此。
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