Matís, Research and Innovation, Vínlandsleið 12, 113, Reykjavík, Iceland.
Trace Element Speciation Laboratory Aberdeen, University of Aberdeen, Meston Walk, Aberdeen, AB24 3UE, UK.
Anal Bioanal Chem. 2019 Jul;411(19):4973-4985. doi: 10.1007/s00216-019-01907-x. Epub 2019 May 31.
Brown macroalgae Saccharina latissima (30-40 individuals) and Alaria esculenta (15-20 individuals) were collected from natural populations in winter in Iceland. The algal thalli were sectioned into different parts (e.g. holdfast, stipe, old frond, young frond and sori-containing frond sections) that differed in age and biological function. The work elucidated that arsenic (As) was not uniformly distributed within the two brown macroalgal species, with lower levels of total As were found in the stipe/midrib compared to other thallus parts. The arsenosugars mirrored the total arsenic in the seaweed mainly due to AsSugSO being the most abundant As species. However, arsenic speciation using parallel HPLC-ICP-MS/ESI-MS elucidated that the arsenic-containing lipids (AsL) had a different distribution where the arsenosugarphospholipids (AsPL) differed by approximately a factor of 4 between the sections containing the lowest and highest concentrations of AsPLs. When placing the sections in order of metabolic activity and an estimate of tissue age, there appeared to be a relationship between the activity and AsPLs, with lower levels of AsPLs in oldest parts. This is the first time such a relationship has been shown for AsLs. Hence, by applying sophisticated analytical techniques, it was possible to gain a deeper understanding of arsenolipids in seaweed.
从冰岛冬季的自然种群中收集了褐藻巨藻（Saccharina latissima）（30-40 株）和海蒿子（Alaria esculenta）（15-20 株）。藻体被切成不同的部分（例如固着器、茎、老叶、嫩叶和含孢子叶），这些部分在年龄和生物功能上有所不同。这项工作阐明了砷（As）在这两种褐藻中并不是均匀分布的，与其他藻体部分相比，茎/中脉中的总砷含量较低。由于 AsSugSO 是最丰富的砷物种，因此砷糖与海藻中的总砷主要相似。然而，使用平行 HPLC-ICP-MS/ESI-MS 对砷进行形态分析表明，含砷脂质（AsL）的分布不同，其中砷糖磷脂（AsPL）在含砷量最低和最高的部分之间相差约 4 倍。当按代谢活性和组织年龄估计对部分进行排序时，似乎在活性和 AsPL 之间存在关系，在最古老的部分中 AsPL 的水平较低。这是首次在砷糖脂中显示出这种关系。因此，通过应用复杂的分析技术，有可能更深入地了解海藻中的砷糖脂。
