Snyder R W, Lambrou F H, Williams G A
Arch Ophthalmol. 1987 Sep;105(9):1277-80. doi: 10.1001/archopht.1987.01060090135044.
Intraocular fibrin formation following ocular surgery is a potentially binding problem. Current therapy for this postoperative fibrin response is often ineffective. In the rabbit, we developed a quantitative reproducible model for intraocular fibrin deposition. Using this model, we have tested the efficacy of human tissue plasminogen activator (tPA) in promoting intraocular fibrinolysis. Citrated rabbit plasma (0.2 mL) was injected intracamerally following paracentesis, resulting in fibrin clot formation within three hours. The fibrin clots were stable for four days, and then slowly lysed over the next four days. Approximately 24 hours after clot formation, various concentrations of human tPA were injected intracamerally. The time taken for clot lysis was dose dependent, with 1800 IU of tPA producing clot lysis in three hours. Toxicity, as measured by intraocular pressure, corneal thickness, inflammation, or cataract formation, was minimal.
眼科手术后眼内纤维蛋白形成是一个潜在的棘手问题。目前针对这种术后纤维蛋白反应的治疗方法往往无效。在兔子身上，我们建立了一个用于眼内纤维蛋白沉积的定量可重复模型。利用这个模型，我们测试了人组织型纤溶酶原激活剂（tPA）促进眼内纤维蛋白溶解的功效。在穿刺后向前房内注射枸橼酸化兔血浆（0.2 mL），3小时内会形成纤维蛋白凝块。纤维蛋白凝块在4天内保持稳定，然后在接下来的4天内缓慢溶解。在凝块形成约24小时后，向眼内注射不同浓度的人tPA。凝块溶解所需时间呈剂量依赖性，1800国际单位的tPA可在3小时内使凝块溶解。通过眼压、角膜厚度、炎症或白内障形成来衡量的毒性极小。
