不同基因组大小的脊椎动物物种的细胞荧光法DNA碱基测定

Cytofluorometric DNA base determination in vertebrate species with different genome sizes.

作者信息

Capriglione T, Olmo E, Odierna G, Improta B, Morescalchi A

出版信息

Basic Appl Histochem. 1987;31(2):119-26.

Abstract

The base composition of DNA was studied in 15 amphibian species and 28 reptile species by means of DAPI, a fluorochrome specific for adenine-thymine rich DNA (AT-rich DNA). The results obtained in reptiles and anuran amphibians coincided with biochemical data available for some species. In urodeles, on the contrary, the findings contrasted with biochemical data and suggest that DAPI is unable to stain all the AT-rich DNA in the erythrocytes of these organisms. It is concluded that the method is suitable for studying species with a small genome size, such as reptiles and anuran amphibians, but is not suitable for nuclei with a large genome size and a highly compact chromatin, such as urodele erythrocytes.

摘要

通过使用DAPI（一种对富含腺嘌呤 - 胸腺嘧啶的DNA（富含AT的DNA）具有特异性的荧光染料），对15种两栖动物和28种爬行动物的DNA碱基组成进行了研究。在爬行动物和无尾两栖动物中获得的结果与一些物种现有的生化数据一致。相反，在有尾两栖动物中，这些发现与生化数据形成对比，表明DAPI无法对这些生物体红细胞中所有富含AT的DNA进行染色。得出的结论是，该方法适用于研究基因组较小的物种，如爬行动物和无尾两栖动物，但不适用于基因组较大且染色质高度致密的细胞核，如有尾两栖动物的红细胞。