Chen Lin, Li Wei, Liu Shaojun, Tao Min, Long Yu, Duan Wei, Zhang Chun, Xiao Jun, Qin Qinbo, Luo Kaikun, Liu Jifang, Liu Yun
Key laboratory of Protein Chemistry and Fish Developmental Biology of State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, Hunan, People's Republic of China.
Mol Cell Probes. 2009 Jun-Aug;23(3-4):157-65. doi: 10.1016/j.mcp.2009.03.001. Epub 2009 Mar 28.
With the pair of degenerate primers designed against the conserved regions of HMG-box of Sox gene family, DNA fragments of different sizes were obtained by amplifying the whole genome DNA samples of many animals, including natural fish, artificial hybrid fish, Aves, reptiles, amphibians and hexapods. Each sample was identified by the specific DNA-band pattern formed by the DNA fragments with defined number and size which marked the sample's genetic characteristics. In addition, 50 DNA fragments from 22 kinds of animals were sequenced and comparatively analyzed so as to study their genetic relationships, especially that between artificial hybrids and their original parents. Based on the specific DNA-band pattern and the specific DNA sequence obtained in tissue DNA sample, we established the novel genetic DNA markers derived from the DNA fragments of Sox genes. The present results proved that the novel DNA markers provided fast and accurate markers for different animal phylogenetic branches and that these convenient markers can also distinguish closely related species and hybrids using a single gene family tool.
利用针对Sox基因家族HMG盒保守区设计的一对简并引物，通过扩增包括天然鱼类、人工杂交鱼、鸟类、爬行动物、两栖动物和六足动物在内的多种动物的全基因组DNA样本，获得了不同大小的DNA片段。每个样本通过由具有特定数量和大小的DNA片段形成的特定DNA条带模式进行鉴定，这些片段标记了样本的遗传特征。此外，对来自22种动物的50个DNA片段进行了测序和比较分析，以研究它们的遗传关系，特别是人工杂交种与其原始亲本之间的遗传关系。基于组织DNA样本中获得的特定DNA条带模式和特定DNA序列，我们建立了源自Sox基因DNA片段的新型遗传DNA标记。目前的结果证明，新型DNA标记为不同动物系统发育分支提供了快速准确的标记，并且这些便捷的标记还可以使用单个基因家族工具区分亲缘关系密切的物种和杂交种。
