使用当前工具和资源的 CRISPR 设计最佳实践。
Best practice for CRISPR design using current tools and resources.
机构信息
Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, United Kingdom.
Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, United Kingdom.
出版信息
Methods. 2019 Jul 15;164-165:3-17. doi: 10.1016/j.ymeth.2019.05.019. Epub 2019 May 30.
PMID:31152780
Abstract
Users facing the task of designing gRNAs for a CRISPR-mutagenesis experiment are typically confronted with a large variety of possible tools and existing libraries. Here we examine the design principles for such resources, and suggest a best practice which allows a user to evaluate and effectively use any of the existing CRISPR design tools or genome-wide libraries.
摘要
用户在面临设计 CRISPR 诱变实验的 gRNA 任务时，通常会遇到各种可能的工具和现有的文库。在这里，我们研究了这些资源的设计原则，并提出了一种最佳实践，使用户能够评估和有效利用任何现有的 CRISPR 设计工具或全基因组文库。
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