在基于深共熔溶剂的共溶剂中，通过将青霉素酰化酶固定在磁性纳米晶纤维素上来改善头孢克洛的生物催化作用。
Improving biocatalysis of cefaclor with penicillin acylase immobilized on magnetic nanocrystalline cellulose in deep eutectic solvent based co-solvent.
机构信息
Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou 510640, Guangdong, China.
School of Food Science and Engineering, Foshan University, Foshan 528000, China.
出版信息
Bioresour Technol. 2019 Sep;288:121548. doi: 10.1016/j.biortech.2019.121548. Epub 2019 May 24.
Deep eutectic solvent (DES), has been considered as a new type of green solvent applied in enzymatic systems. Here, we reported DES-buffer co-solvent as a novel reaction medium for high efficient synthesis of cefaclor by penicilin acylase immobilized on magnetic nanocrystalline cellulose. Effect of DES composition, DES-buffer ratio, temperature, pH, substrate ratio and substrate concentration was systematically investigated. In co-solvent consisting of choline chloride (ChCl):glycol-buffer (7:3, v/v), conversion of 7-ACCA was 94%, synthesis to hydrolysis ratio was 1.8, and yield of cefaclor reached 91%, higher than that in aqueous buffer with optimized yield of 84%, showing the great potential of DES as organic solvent alternative. To the best of our knowledge, this is the first example of biosynthesis of cefaclor in the DES-buffer co-solvent.
深共熔溶剂（DES）已被认为是一种新型的绿色溶剂，应用于酶系统中。在这里，我们报道了 DES-缓冲共溶剂作为一种新型的反应介质，用于通过固定在磁性纳米结晶纤维素上的青霉素酰化酶高效合成头孢克洛。系统研究了 DES 组成、DES-缓冲比、温度、pH 值、底物比和底物浓度的影响。在由氯化胆碱（ChCl）：甘醇-缓冲液（7：3，v/v）组成的共溶剂中，7-ACCA 的转化率为 94%，合成到水解的比例为 1.8，头孢克洛的收率达到 91%，高于优化收率为 84%的水缓冲液，表明 DES 作为有机溶剂替代品具有巨大的潜力。据我们所知，这是 DES-缓冲共溶剂中生物合成头孢克洛的第一个例子。
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