Guangdong Province Key Laboratory of Stomatology, Guangzhou, Guangdong, China; Department of Operative Dentistry and Endodontics, Guanghua School and Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China.
Guangdong Province Key Laboratory of Stomatology, Guangzhou, Guangdong, China; Zhujiang New Town Dental Clinic, Guanghua School and Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China.
J Endod. 2019 Jul;45(7):890-897. doi: 10.1016/j.joen.2019.03.018. Epub 2019 May 29.
The aim was to assess the association of inducible costimulator (ICOS) and ICOS ligand with bone destruction in apical periodontitis (AP).
Specimens from patients presenting with AP were obtained during apicoectomy and subjected to histopathologic analysis and molecular assessment of ICOS/ICOS ligand. In addition, the experimental AP was induced by exposing the pulp of first mandibular molars of rats. Histologic and radiographic examinations were performed to validate the periapical lesions. The immunolocalization and messenger RNA expression of ICOS/ICOS ligand were evaluated by immunofluorescence staining and quantitative real-time polymerase chain reaction. The osteoclastic activities in periapical lesions, including the lesion size and the expression of tartrate-resistant acid phosphatase and the receptor activator of nuclear factor kappa B ligand, were recorded and followed by correlation analysis with ICOS/ICOS ligand expression.
In excisional specimens from AP patients, a significantly increased expression of ICOS/ICOS ligand was found compared with the healthy control. In the experimental AP samples, the expression of ICOS/ICOS ligand, tartrate-resistant acid phosphatase, and receptor activator of nuclear factor kappa B ligand was significantly elevated in inflamed periapical tissues (AP group) when compared with the healthy control. The number of ICOS/ICOS ligand cells was highly correlated with the periapical lesion size (r = 0.892, P < .01 and r = 0.930, P < .01, respectively).
The increased expression of ICOS/ICOS ligand in periapical lesions was associated with the inflammatory infiltration and alveolar bone destruction of AP.
目的是评估诱导共刺激分子（ICOS）及其配体与根尖周病（AP）骨破坏的关系。
在根尖切除术期间获得患有 AP 的患者的标本，并进行组织病理学分析和 ICOS/ICOS 配体的分子评估。此外，通过暴露大鼠第一下颌磨牙的牙髓来诱导实验性 AP。进行组织学和放射学检查以验证根尖周病变。通过免疫荧光染色和定量实时聚合酶链反应评估 ICOS/ICOS 配体的免疫定位和信使 RNA 表达。记录根尖病变中的破骨细胞活性，包括病变大小以及抗酒石酸酸性磷酸酶和核因子 kappa B 受体激活剂配体的表达，并与 ICOS/ICOS 配体表达进行相关性分析。
与健康对照组相比，AP 患者的切除标本中发现 ICOS/ICOS 配体的表达显著增加。在实验性 AP 样本中，与健康对照组相比，炎症性根尖组织（AP 组）中 ICOS/ICOS 配体、抗酒石酸酸性磷酸酶和核因子 kappa B 受体激活剂配体的表达显著升高。ICOS/ICOS 配体细胞的数量与根尖病变大小高度相关（r = 0.892，P <.01 和 r = 0.930，P <.01）。
根尖周病变中 ICOS/ICOS 配体的表达增加与 AP 的炎症浸润和牙槽骨破坏有关。
