大鼠根尖周病变中肿瘤坏死因子受体相关因子6的免疫定位
Immunolocalization of tumor necrosis factor receptor-associated factor 6 in rat periapical lesions.
作者信息
Yang Lijie, Zhang Chi, Peng Bin
机构信息
Department of Operative Dentistry and Endodontics, School and Hospital of Stomatology, Wuhan University, China.
出版信息
J Endod. 2009 Jun;35(6):834-7. doi: 10.1016/j.joen.2009.03.011.
INTRODUCTION
Alveolar bone destruction is associated with enhanced osteoclast activity; tumor necrosis factor receptor-associated factor 6 (TRAF6) is suggested to contribute to the differentiation of osteoclasts. The purpose of this study was to observe the immunohistochemical localization of TRAF6 and bone resorption in induced periapical lesions in rats.
METHODS
Periapical lesions were induced by an occlusal pulp exposure in the mandibular first molars. Animals were randomly killed at 0, 7, 14, 21, and 28 days. Frontal sections were prepared for immunohistochemistry and enzyme histochemistry.
RESULTS
A few TRAF6-positive cells and osteoclasts could be observed on day 7. Both climaxed on day 14. In the 21-day and 28-day samples, TRAF6 expression decreased, and fewer osteoclasts were observed. From day 7 to day 28, a significant positive correlation was found between TRAF6 expression and osteoclast numbers.
CONCLUSIONS
These findings showed that TRAF6 might be associated with osteoclast differentiation in periapical lesions.
引言
牙槽骨破坏与破骨细胞活性增强有关；肿瘤坏死因子受体相关因子6（TRAF6）被认为有助于破骨细胞的分化。本研究的目的是观察TRAF6在大鼠诱导性根尖周病变中的免疫组化定位及骨吸收情况。
方法
通过下颌第一磨牙咬合面牙髓暴露诱导根尖周病变。在第0、7、14、21和28天随机处死动物。制备额状切片用于免疫组化和酶组织化学。
结果
在第7天可观察到少量TRAF6阳性细胞和破骨细胞。两者在第14天达到高峰。在21天和28天的样本中，TRAF6表达下降，观察到的破骨细胞较少。从第7天到第28天，TRAF6表达与破骨细胞数量之间存在显著正相关。
结论
这些发现表明TRAF6可能与根尖周病变中的破骨细胞分化有关。
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