Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of São José dos Campos, Sao Paulo State University (Unesp), Av. Engenheiro Francisco José Longo, 777, Sao Jose dos Campos, Sao Paulo, 12245-000, Brazil.
Department of Dental Materials and Prosthodontics, Institute of Science and Technology of São José dos Campos, Sao Paulo State University (Unesp), Sao Jose dos Campos, SP, Brazil.
Clin Oral Investig. 2020 Feb;24(2):785-797. doi: 10.1007/s00784-019-02946-5. Epub 2019 Jun 1.
Evaluate the modulating effect of ionizing radiation, blood cytokine levels, and bone remodeling of the interface around the implant to understand the radiation mechanisms which can impair the implants receptor site.
Sixty rats were submitted to grade V titanium implants in the femurs and were divided into the following groups: no-irradiation (N-Ir): control group with implant only; early-irradiation (E-Ir): implant + irradiation after 24 h; late-irradiation (L-Ir): implant + irradiation after 4 weeks; and previous-irradiation (P-Ir): irradiation + implant after 4 weeks. The animals in the E-Ir, L-Ir, and P-Ir groups were irradiated in two fractional stages of 15 Gy. At 3 days, 2 weeks, and 7 weeks after the final procedure, five animals were randomly euthanized per group. Serum levels of TNF-ɑ, IL-1β, TGF-β, IL-6, M-CSF, and IL-10 were measured from blood collected prior to euthanasia using the ELISA test. The pieces containing the implants were subjected to immunohistochemical labeling using the tartrate acid resistant to phosphatase, osteocalcin, and caspase-3 markers and mCT. The ANOVA test was used for statistical analysis, and the Tukey multiple comparison test (p < 0.05) was applied.
The results indicated that ionizing radiation modifies the production of pro- and anti-inflammatory serum cytokines, the expression of proteins involved in bone remodeling and cellular apoptosis, as well as changes in bone formation.
The results suggests that a longer period between radiotherapy and implant placement surgery when irradiation occurs prior to implant installation would allow the recovery and renewal of bone cells and avoid future failures in osseointegration.
The search for modifications caused by ionizing irradiation in bone tissue can indicate the ideal period for implant placement without affecting the osseointegration process.
评估电离辐射、血液细胞因子水平和植入物周围界面的骨重塑对理解损害植入物受体部位的辐射机制的调节作用。
60 只大鼠股骨中植入 V 级钛植入物，并分为以下几组：无照射（N-Ir）：仅植入物对照；早期照射（E-Ir）：植入后 24 小时照射；晚期照射（L-Ir）：植入后 4 周照射；和预先照射（P-Ir）：照射后 4 周植入。E-Ir、L-Ir 和 P-Ir 组中的动物接受两次 15Gy 的分次照射。在最后一次手术后 3 天、2 周和 7 周时，每组随机处死 5 只动物。在安乐死前从血液中用 ELISA 法测量 TNF-ɑ、IL-1β、TGF-β、IL-6、M-CSF 和 IL-10 的血清水平。用抗酒石酸酸性磷酸酶、骨钙素和 caspase-3 标记物和 mCT 对含植入物的标本进行免疫组织化学标记。采用方差分析进行统计学分析，并用 Tukey 多重比较检验（p<0.05）进行检验。
结果表明，电离辐射改变了促炎和抗炎血清细胞因子的产生、参与骨重塑和细胞凋亡的蛋白表达，以及骨形成的变化。
结果表明，在植入物安装前进行放射治疗和植入物放置手术之间的时间间隔更长，将允许骨细胞的恢复和更新，避免未来的骨整合失败。
寻找骨组织中电离辐射引起的变化可以指示植入物放置的理想时期，而不会影响骨整合过程。
