Leventis Minas, Fairbairn Peter, Mangham Chas, Galanos Antonios, Vasiliadis Orestis, Papavasileiou Danai, Horowitz Robert
Laboratory of Experimental Surgery and Surgical Research N. S. Christeas, Medical School, University of Athens, 75 M. Assias Street, Athens 115 27, Greece.
Department of Periodontology and Implant Dentistry, School of Dentistry, University of Detroit Mercy, 2700 Martin Luther King Jr Boulevard, Detroit, MI 48208, USA.
Materials (Basel). 2018 Oct 17;11(10):2004. doi: 10.3390/ma11102004.
Bioactive alloplastic materials, like beta-tricalcium phosphate (β-TCP) and calcium sulfate (CS), have been extensively researched and are currently used in orthopedic and dental bone regenerative procedures. The purpose of this study was to compare the performance of EthOss versus a bovine xenograft and spontaneous healing. The grafting materials were implanted in standardized 8 mm circular bicortical bone defects in rabbit calvariae. A third similar defect in each animal was left empty for natural healing. Six male rabbits were used. After eight weeks of healing, the animals were euthanized and the bone tissue was analyzed using histology and micro-computed tomography (micro-CT). Defects treated with β-TCP/CS showed the greatest bone regeneration and graft resorption, although differences between groups were not statistically significant. At sites that healed spontaneously, the trabecular number was lower ( < 0.05) and trabecular separation was higher ( < 0.05), compared to sites treated with β-TCP/CS or xenograft. Trabecular thickness was higher at sites treated with the bovine xenograft ( < 0.05) compared to sites filled with β-TCP/CS or sites that healed spontaneously. In conclusion, the novel β-TCP/CS grafting material performed well as a bioactive and biomimetic alloplastic bone substitute when used in cranial defects in this animal model.
生物活性异质材料，如β-磷酸三钙（β-TCP）和硫酸钙（CS），已得到广泛研究，目前用于骨科和牙科的骨再生手术。本研究的目的是比较EthOss与牛异种移植物以及自发愈合的性能。将移植材料植入兔颅骨标准化的8毫米圆形双皮质骨缺损处。每只动物留出第三个类似的缺损处不进行处理，让其自然愈合。使用了六只雄性兔子。愈合八周后，对动物实施安乐死，并使用组织学和微型计算机断层扫描（微型CT）对骨组织进行分析。尽管各组之间的差异无统计学意义，但用β-TCP/CS处理的缺损处显示出最大程度的骨再生和移植物吸收。与用β-TCP/CS或异种移植物处理的部位相比，自发愈合部位的骨小梁数量较少（<0.05），骨小梁间距较大（<0.05）。与填充β-TCP/CS的部位或自发愈合的部位相比，用牛异种移植物处理的部位骨小梁厚度更高（<0.05）。总之，在该动物模型中，新型β-TCP/CS移植材料作为一种生物活性和仿生异质骨替代物用于颅骨缺损时表现良好。
