de Araújo Juliani Caroline Ribeiro, Sobral Silva Leonardo Alvares, de Barros Lima Vinicius Almeida, Bastos Campos Tiago Moreira, Lisboa Filho Paulo Noronha, Okamoto Roberta, de Vasconcellos Luana Marotta Reis
Department of Bioscience and Buccal Diagnose, Institute of Science and Technology, Sao Paulo State University, UNESP, Sao Paulo 12245-700, SP, Brazil.
Department of Prosthodontics and Periodontology, Bauru School of Dentistry, University of Sao Paulo, Bauru 17012-230, SP, Brazil.
J Funct Biomater. 2023 Feb 9;14(2):93. doi: 10.3390/jfb14020093.
With the increase in the population's life expectancy, there has also been an increase in the rate of osteoporosis, which has expanded the search for strategies to regenerate bone tissue. The ultrasonic sonochemical technique was chosen for the functionalization of the 45S5 bioglass. The samples after the sonochemical process were divided into (a) functionalized bioglass (BG) and (b) functionalized bioglass with 10% teriparatide (BGT). Isolated mesenchymal cells (hMSC) from femurs of ovariectomized rats were differentiated into osteoblasts and submitted to in vitro tests. Bilateral ovariectomy (OVX) and sham ovariectomy (Sham) surgeries were performed in fifty-five female Wistar rats. After a period of 60 days, critical bone defects of 5.0 mm were created in the calvaria of these animals. For biomechanical evaluation, critical bone defects of 3.0 mm were performed in the tibias of some of these rats. The groups were divided into the clot (control) group, the BG group, and the BGT group. After the sonochemical process, the samples showed modified chemical topographic and morphological characteristics, indicating that the surface was chemically altered by the functionalization of the particles. The cell environment was conducive to cell adhesion and differentiation, and the BG and BGT groups did not show cytotoxicity. In addition, the experimental groups exhibited characteristics of new bone formation with the presence of bone tissue in both periods, with the BGT group and the OVX group statistically differing from the other groups ( < 0.05) in both periods. Local treatment with the drug teriparatide in ovariectomized animals promoted positive effects on bone tissue, and longitudinal studies should be carried out to provide additional information on the biological performance of the mutual action between the bioglass and the release of the drug teriparatide.
随着人口预期寿命的增加，骨质疏松症的发病率也有所上升，这促使人们寻求骨组织再生策略。选择超声声化学技术对45S5生物玻璃进行功能化处理。声化学处理后的样品分为(a)功能化生物玻璃（BG）和(b)含10%特立帕肽的功能化生物玻璃（BGT）。从去卵巢大鼠股骨中分离的间充质细胞（hMSC）分化为成骨细胞并进行体外测试。对55只雌性Wistar大鼠进行双侧卵巢切除术（OVX）和假卵巢切除术（Sham）。60天后，在这些动物的颅骨上制造5.0 mm的临界骨缺损。为进行生物力学评估，在其中一些大鼠的胫骨上制造3.0 mm的临界骨缺损。将这些组分为血凝块（对照）组、BG组和BGT组。声化学处理后，样品显示出化学形貌和形态特征的改变，表明颗粒功能化使表面发生了化学变化。细胞环境有利于细胞黏附和分化，BG组和BGT组未表现出细胞毒性。此外，实验组在两个时期均表现出新骨形成的特征，骨组织存在，BGT组和OVX组在两个时期与其他组相比差异有统计学意义（<0.05）。在去卵巢动物中局部使用药物特立帕肽对骨组织产生了积极影响，应开展纵向研究以提供有关生物玻璃与药物特立帕肽释放之间相互作用的生物学性能的更多信息。
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