Department of Pharmacology & Toxicology, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, 281001, India.
Cardiovasc Toxicol. 2020 Feb;20(1):28-48. doi: 10.1007/s12012-019-09531-y.
The present study was designed to assess the cardio-protective role of oleic acid in myocardial injury (MI) induced by intra-peritoneal injection of isoprenaline (ISO) in rats for 2 consecutive days. Oleic acid (OA) was administered orally (@ 5 mg/kg b.wt and 10 mg/kg b.wt) for 21 days before inducing MI. Pre-exposure to OA at higher dose significantly improved the HW/BW ratio, myocardial infarct size, lipid profiles (total cholesterol, HDL-C) and cardiac injury biomarkers (LDH, CK-MB, cardiac troponin-I, MMP-9), thus suggesting its cardio-protective role. The ameliorative potential of the higher dose of OA was further substantiated by its ability to reduce the cardiac oxidative stress as evidenced by significant decrease in lipid peroxidation coupled with increase in superoxide dismutase activity and reduced glutathione level. Significant decrease in heart rate as well as increase in RR and QT intervals in oleic acid pre-exposed rats were also observed. OA pre-treatment also reduced the histopathological alterations seen in myocardial injury group rats. The mRNA expression of cardiac UCP-2 gene, a regulator of reactive oxygen species (ROS) generation, was significantly increased in oleic acid pre-exposure group compared to the ISO-induced myocardial injury group. Thus increase in expression of UCP-2 gene in cardiac tissue seems to be one of the protective measures against myocardial injury. Based on the above findings, it may be inferred that oleic acid possesses promising cardio-protective potential against myocardial injury due to its anti-oxidative property and ability to modulate cardiac metabolic processes.
本研究旨在评估油酸在腹腔注射异丙肾上腺素（ISO）连续 2 天诱导大鼠心肌损伤（MI）中的心脏保护作用。油酸（OA）在诱导 MI 前口服给药（@ 5mg/kg bw 和 10mg/kg bw）21 天。预先暴露于高剂量 OA 可显著改善 HW/BW 比、心肌梗死面积、血脂谱（总胆固醇、HDL-C）和心脏损伤生物标志物（LDH、CK-MB、心肌肌钙蛋白 I、MMP-9），从而表明其心脏保护作用。高剂量 OA 的改善潜力还通过其降低心脏氧化应激的能力得到进一步证实，表现为脂质过氧化显著减少，同时超氧化物歧化酶活性和还原型谷胱甘肽水平增加。在油酸预先暴露的大鼠中还观察到心率显著降低，RR 和 QT 间隔增加。OA 预处理还减少了心肌损伤组大鼠的组织病理学改变。与 ISO 诱导的心肌损伤组相比，油酸预先暴露组心脏 UCP-2 基因（一种活性氧（ROS）生成调节剂）的 mRNA 表达显著增加。因此，心脏组织中 UCP-2 基因表达的增加似乎是一种针对心肌损伤的保护措施。基于上述发现，可以推断油酸具有有前途的心脏保护潜力，可预防心肌损伤，这与其抗氧化特性和调节心脏代谢过程的能力有关。
