Department of Microbiology, College of Basic Medicine, Zunyi Medical University, Huichuan District, Zunyi, 563003, Guizhou, China.
College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China.
Environ Sci Pollut Res Int. 2019 Aug;26(22):22305-22311. doi: 10.1007/s11356-019-05429-y. Epub 2019 Jun 1.
The dissemination of extended spectrum beta-lactamases (ESBL) genes through gene transfer attracts wide attention. Bentonite is widely used as a feed additive in an animal-breeding environment. In order to obtain a better understanding of the effect of bentonite on Escherichia coli carrying ESBL gene, proteomic analysis was carried out to screen the key proteins. The results showed that a total of 31 proteins were differentially expressed, including 21 up-regulated proteins and 10 down-regulated proteins. These proteins were involved in biosynthetic process, metabolic process, stress response, transport, anaerobic respiration, proteolysis, hydrolase, protein folding, transcription, salvage, and other. The transcriptional level of four genes (mipA, gntY, tldD, and arcA) was in consensus with proteomic results. This study revealed the differentially expressed proteins involved when E. coli was incubated under bentonite and PBS condition, which implied the possibility that bentonite may promote the transfer of ESBL gene between E. coli.
通过基因转移传播扩展谱β-内酰胺酶（ESBL）基因引起了广泛关注。膨润土作为一种饲料添加剂广泛应用于动物养殖环境中。为了更好地了解膨润土对携带 ESBL 基因的大肠杆菌的影响，进行了蛋白质组学分析以筛选关键蛋白。结果表明，共有 31 种蛋白差异表达，其中 21 种上调蛋白和 10 种下调蛋白。这些蛋白参与生物合成过程、代谢过程、应激反应、运输、厌氧呼吸、蛋白水解、水解酶、蛋白折叠、转录、挽救等。四个基因（mipA、gntY、tldD 和 arcA）的转录水平与蛋白质组学结果一致。本研究揭示了当大肠杆菌在膨润土和 PBS 条件下孵育时涉及的差异表达蛋白，这表明膨润土可能促进 ESBL 基因在大肠杆菌之间的转移。
