Advanced Biomass R&D Center (ABC), KAIST, Daejeon 34141, Republic of Korea.
Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
J Microbiol Biotechnol. 2019 Jun 28;29(6):952-961. doi: 10.4014/1904.04008.
spp. are green algae that are found across wide-ranging habitats from deserts to arctic regions, with various strains having adapted to survive under diverse environmental conditions. In this study, two novel strains (ABC-002, ABC-008) were isolated from a freshwater lake in South Korea during the winter season and examined for possible use in the biofuel production process. The comparison of ABC-002 and ABC-008 strains with UTEX265 under two different temperatures (10°C, 25°C) revealed their cold-tolerant phenotypes as well as high biomass yields. The maximum quantum yields of UTEX25, ABC- 002, and ABC-008 at 10°C were 0.5594, 0.6747, and 0.7150, respectively, providing evidence of the relatively higher cold-resistance capabilities of these two strains. Furthermore, both the biomass yields and lipid content of the two novel strains were found to be higher than those of UTEX265; the overall lipid productivities of ABC-002 and ABC-008 were 1.7 ~ 2.8 fold and 1.6 ~ 4.2 fold higher compared to that of UTEX265, respectively. Thus, the high biomass and lipid productivity over a wide range of temperatures indicate that ABC-002 and ABC-008 are promising candidates for applications in biofuel productions via outdoor biomass cultivation.
spp. 是一种绿色藻类，广泛分布于从沙漠到北极地区等各种生境中，各种菌株已经适应了在不同的环境条件下生存。在这项研究中，从韩国冬季的一个淡水湖中分离出了两种新型菌株（ABC-002、ABC-008），并研究了它们在生物燃料生产过程中的可能用途。在两种不同温度（10°C、25°C）下，将 ABC-002 和 ABC-008 菌株与 UTEX265 进行比较，发现它们具有耐寒表型和高生物质产量。在 10°C 时，UTEX25、ABC-002 和 ABC-008 的最大量子产量分别为 0.5594、0.6747 和 0.7150，这证明了这两种菌株具有相对较高的耐寒能力。此外，两种新型菌株的生物质产量和脂质含量均高于 UTEX265；ABC-002 和 ABC-008 的总脂生产力分别比 UTEX265 高 1.72.8 倍和 1.64.2 倍。因此，高生物质和脂质生产力以及宽温度范围表明，ABC-002 和 ABC-008 是通过户外生物质培养应用于生物燃料生产的有前途的候选者。
