Zhang Zhenyu, Zhang Xiaoli, Wu Yinqiang, Yao Li-Hua, Fu Pengcheng
School of Life Science, Jiangxi Science & Technology Normal University, Nanchang 330013, China.
State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China.
Microorganisms. 2025 Mar 21;13(4):711. doi: 10.3390/microorganisms13040711.
is a rich source of nutrients. In addition to its nutritional value, it exhibits versatile biological activities. New strains have been extensively identified and investigated in recent years to expand the potential of . The accurate measurement of pulse-amplitude-modulated (PAM) fluorometry parameters and effective microalgal cell lysis are foundational for advanced studies of novel species. In this study, ribosomal small subunit (SSU)-internal transcribed spacer (ITS) phylogenetic analysis and internal transcribed spacer 2 (ITS2) secondary structure analysis were employed to identify a new species. The dark adaptation time, the duration of the saturation pulse, the intensity of actinic light, and the duration of actinic light exposure for PAM fluorometry measurements were optimized. Different conditions of ultrasonication and high-pressure homogenization (HPH) for microalgal cell lysis were compared. K-01 was identified. The suitable duration for dark adaptation, the saturation pulse, and the actinic light were 15 min, 200 milliseconds, and 30 s, respectively. The suitable intensity of actinic light was 191 μE/(m·s). For microalgal cell lysis, HPH could achieve 98.65% cell lysis efficiency at 30 kpsi (207 MPa), whereas ultrasonication attained an efficiency of 45.47% (300 W for 30 min). These results facilitate further study on the physiology and the composition of K-01.
是丰富的营养来源。除了其营养价值外，它还具有多种生物活性。近年来，为了扩大……的潜力，已经广泛鉴定和研究了新菌株。脉冲幅度调制（PAM）荧光测定参数的准确测量和有效的微藻细胞裂解是新型……物种深入研究的基础。在本研究中，采用核糖体小亚基（SSU）-内转录间隔区（ITS）系统发育分析和内转录间隔区2（ITS2）二级结构分析来鉴定一种新的……物种。对PAM荧光测定的暗适应时间、饱和脉冲持续时间、光化光强度和光化光暴露持续时间进行了优化。比较了微藻细胞裂解的不同超声处理和高压均质化（HPH）条件。鉴定出了K-01。暗适应、饱和脉冲和光化光的合适持续时间分别为15分钟、200毫秒和30秒。光化光的合适强度为191μE/（m·s）。对于微藻细胞裂解，HPH在30 kpsi（207 MPa）下可实现98.65%的细胞裂解效率，而超声处理的效率为45.47%（300 W处理30分钟）。这些结果有助于对K-01的生理学和组成进行进一步研究。
