Department of Microbiology, Prince of Wales Hospital, Shatin, Hong Kong Special Administrative Region, China.
Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Kowloon, Hong Kong Special Administrative Region, China.
Microb Drug Resist. 2019 Oct;25(8):1199-1203. doi: 10.1089/mdr.2018.0433. Epub 2019 Jun 3.
The aim of the study was to analyze the epidemiology of and investigate the genetic characteristics of carbapenem-resistant (CRAB) isolates isolated from blood cultures in a regional hospital in Hong Kong. Twenty blood culture isolates were collected from a regional hospital in Hong Kong from 2014 to 2017. Twenty isolates were grouped into five existing sequence types (STs) and five new STs within the following prevalence: ST195 was predominant with a prevalence of 45% ( = 9), followed by ST373 and ST447 (10%; = 2 each), and ST176 and ST345 (5%; = 1 each). Resistance to carbapenem antibiotics was 55% ( = 11). Six carbapenem-resistant isolates harbored genes and IS mobile elements. Polymerase chain reaction confirmed that IS1 is located upstream to the genes, suggesting an association between IS and genes with carbapenem resistance. This study is the first to report the emergence of CRAB ST195 harboring in Hong Kong.
本研究旨在分析 的流行病学，并调查从香港一家地区医院血培养中分离出的耐碳青霉烯类 （CRAB）分离株的遗传特征。 从 2014 年至 2017 年，从香港一家地区医院采集了 20 株血培养分离株。20 株分离株分为五个现有序列类型（ST）和五个新 ST，流行率如下：ST195 占优势，流行率为 45%（ = 9），其次是 ST373 和 ST447（10%； = 2 株），ST176 和 ST345（5%； = 1 株）。对碳青霉烯类抗生素的耐药率为 55%（ = 11）。6 株耐碳青霉烯类分离株携带 基因和 IS 移动元件。聚合酶链反应证实 IS1 位于 基因上游，提示 IS 与 基因与碳青霉烯类耐药之间存在关联。 本研究首次报道了香港出现携带 基因的 CRAB ST195。
