a International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology of Hunan Province, Central South University of Forestry & Technology , 498 Southern Shaoshan Road, Changsha 410004 , China.
b Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry & Technology , Changsha 410004 , China.
Mycologia. 2019 Jul-Aug;111(4):529-540. doi: 10.1080/00275514.2019.1615816. Epub 2019 Jun 3.
Recent progress has been made in adding exogenous vegetable oils in culture media to promote bioactive metabolite production in several medicinal mushrooms, but the mechanism is still unclear. In this study, we found that the vegetable oil coix seed oil (CSO) could induce the biosynthesis of triterpene acids (TAs) and also significantly increase cytoplasmic nitric oxide (NO) and hydrogen peroxide (HO) concentrations in the mycelium of . The change in TA biosynthesis caused by CSO could be reversed by adding NO scavenger or HO scavenger, and adding NO scavenger or HO scavenger resulted in the reduction of the cytoplasmic HO or NO concentration under CSO treatment, respectively. Moreover, adding NO scavenger or HO scavenger reversed TA biosynthesis, which could be rescued by HO or NO donor, respectively. Taken together, our study indicated that both NO and HO were involved in the regulation of TA biosynthesis, and CSO-activated NO and HO were interdependent but independently regulated the TA biosynthesis under CSO treatment in .
近年来，在培养基中添加外源植物油以促进几种药用蘑菇中生物活性代谢产物的生产方面取得了进展，但作用机制仍不清楚。在这项研究中，我们发现植物油薏苡仁油（CSO）可以诱导三萜酸（TAs）的生物合成，并且还可以显著增加菌丝体细胞质中一氧化氮（NO）和过氧化氢（HO）的浓度。CSO 引起的 TA 生物合成的变化可以通过添加 NO 清除剂或 HO 清除剂来逆转，并且在 CSO 处理下添加 NO 清除剂或 HO 清除剂分别导致细胞质 HO 或 NO 浓度降低。此外，添加 NO 清除剂或 HO 清除剂逆转 TA 生物合成，分别可以通过 HO 或 NO 供体来挽救。总之，我们的研究表明，NO 和 HO 都参与了 TA 生物合成的调节，并且 CSO 激活的 NO 和 HO 相互依存，但独立调节 CSO 处理下的 TA 生物合成。
