Interdependent nitric oxide and hydrogen peroxide independently regulate the coix seed oil-induced triterpene acid accumulation in .

Recent progress has been made in adding exogenous vegetable oils in culture media to promote bioactive metabolite production in several medicinal mushrooms, but the mechanism is still unclear. In this study, we found that the vegetable oil coix seed oil (CSO) could induce the biosynthesis of triterpene acids (TAs) and also significantly increase cytoplasmic nitric oxide (NO) and hydrogen peroxide (HO) concentrations in the mycelium of . The change in TA biosynthesis caused by CSO could be reversed by adding NO scavenger or HO scavenger, and adding NO scavenger or HO scavenger resulted in the reduction of the cytoplasmic HO or NO concentration under CSO treatment, respectively. Moreover, adding NO scavenger or HO scavenger reversed TA biosynthesis, which could be rescued by HO or NO donor, respectively. Taken together, our study indicated that both NO and HO were involved in the regulation of TA biosynthesis, and CSO-activated NO and HO were interdependent but independently regulated the TA biosynthesis under CSO treatment in .