Fang Fang, Dai Chuanchao, Wang Yu
College of Life Science, Nanjing Normal University, Nanjing 210046, China.
Sheng Wu Gong Cheng Xue Bao. 2009 Oct;25(10):1490-6.
Crude elicitor of one endophytic fungi (belong to Cunninghamella sp., named AL4) induced multiple responses in Atractylodes lancea suspension cells, including rapid generation of nitric oxide (NO) and hydrogen peroxide (H2O2), sequentially followed by enhancement of essential oil production. Adding NO-specific scavenger 2-4-carboxyphenyl-4,4,5, 5-tetramethylimidazol ine-1-oxyl-3-oxide (cPTIO) and H2O2 scavenger catalase (CAT) could block elicitor-induced NO and H2O2 generation respectively, but could all partly block elicitor-induced essential oil biosynthesis. Adding NO-donor sodium nitroprusside (SNP) and H2O2 could all promote essential oil accumulation in A. lancea cells, but the effect of both was different. These results strongly suggested that NO and H2O2 may all act as signaling molecule to mediate AL4 elicitor promoting essential oil accumulation in suspension cells of A. lancea. Furthermore, adding cPTIO and CAT contemporarily could not completely inhibit essential oil accumulation induced by AL4 elicitor. This result suggested that AL4 elicitor could also promote essential oil accumulation in suspension cells of A. lancea by other means.
一种内生真菌（属于小克银汉霉属，命名为AL4）的粗提激发子可诱导茅苍术悬浮细胞产生多种反应，包括一氧化氮（NO）和过氧化氢（H2O2）的快速生成，随后依次是精油产量的提高。添加NO特异性清除剂2-（4-羧基苯基）-4,4,5,5-四甲基咪唑啉-1-氧基-3-氧化物（cPTIO）和H2O2清除剂过氧化氢酶（CAT）可分别阻断激发子诱导的NO和H2O2生成，但均可部分阻断激发子诱导的精油生物合成。添加NO供体硝普钠（SNP）和H2O2均可促进茅苍术细胞中精油的积累，但二者的效果有所不同。这些结果强烈表明，NO和H2O2可能均作为信号分子介导AL4激发子促进茅苍术悬浮细胞中精油的积累。此外，同时添加cPTIO和CAT不能完全抑制AL4激发子诱导的精油积累。该结果表明，AL4激发子还可通过其他方式促进茅苍术悬浮细胞中精油的积累。
