Green Michael R, Sambrook Joseph
Cold Spring Harb Protoc. 2019 Jun 3;2019(6):2019/6/pdb.prot095224. doi: 10.1101/pdb.prot095224.
Polymerase chain reaction (PCR), rather than the more labor-intensive minipreps, is often used to screen colonies of for recombinant plasmids containing sequences of interest. When screening colonies for a DNA fragment amplified by PCR, the same primers used in the amplification reaction can be used for screening. To discover the orientation of the cloned fragment, a third primer can be used that binds to the plasmid sequences immediately adjacent to the insertion site and points toward the insert.
