Shirshikov F V, Bespyatykh J A
Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia.
Russ J Bioorg Chem. 2022;48(6):1159-1174. doi: 10.1134/S106816202206022X. Epub 2022 Dec 23.
Increasing the accuracy of pathogen identification and reducing the duration of analysis remain relevant for modern molecular diagnostics up to this day. In laboratory and clinical practice, detection of pathogens mostly relies on methods of nucleic acid amplification, among which the polymerase chain reaction (PCR) is considered the "gold standard." Nevertheless, in some cases, isothermal amplification methods act as an alternative to PCR diagnostics. Upon more than thirty years of the development of isothermal DNA synthesis, the appearance of loop-mediated isothermal amplification (LAMP) has enabled new directions of in-field diagnostics of bacterial and viral infections. This review examines the key characteristics of the LAMP method and corresponding features in practice. We discuss the structure of LAMP amplicons with single-stranded loops, which have the sites for primer annealing under isothermal conditions. The latest achievements in the modification of the LAMP method are analyzed, which allow considering it as a unique platform for creating the next-generation diagnostic assays.
提高病原体鉴定的准确性并缩短分析时间至今仍是现代分子诊断领域的重要课题。在实验室和临床实践中，病原体检测主要依赖核酸扩增方法，其中聚合酶链反应（PCR）被视为“金标准”。然而，在某些情况下，等温扩增方法可作为PCR诊断的替代方法。经过三十多年的等温DNA合成技术发展，环介导等温扩增技术（LAMP）的出现为细菌和病毒感染的现场诊断开辟了新方向。本综述探讨了LAMP方法的关键特性及实际应用中的相应特点。我们讨论了具有单链环的LAMP扩增子结构，这些单链环在等温条件下具有引物退火位点。分析了LAMP方法改进方面的最新成果，这使得该方法可被视为创建下一代诊断检测的独特平台。
