Department of Life Sciences, University of Siena, Via A. Moro 2, 53100, Siena, Italy.
Department of Biology, Ecology and Earth Sciences, Di.B.E.S.T., University of Calabria, Via P. Bucci, Cubo 4B, 87036, Rende, Italy.
Arthropod Struct Dev. 2019 Jul;51:1-13. doi: 10.1016/j.asd.2019.05.001. Epub 2019 Jun 14.
Sperm structure of seven species from different Carabidae tribes was studied. Carabus preslii and Carabus granulatus interstitialis form sperm bundles (spermatozeugmata) in which the heads of conventional insect sperm cells are embedded in the apical cap (spermatostyle), leaving the posterior flagella free. On the contrary, Pterostichus morio, Pterostichus melas, Pterostichus melanarius and Amara aulica form complex spermatozeugmata bearing conspicuous axial spermatostyles associated with lateral groups of sperm. Individual sperm cells are contained in chambers delimited by laminar extensions of the cortical region of the spermatostyle. Demetrias atricapillus shares the general spermatozeugma structure with the above species, but the anterior spermatostyle region has a different structure and posteriorly, the lateral groups of sperm are separated from the spermatostyle but remain connected to it by peduncles consisting of extensions of the cortical region of the spermatostyle. The sperm of the species examined in the study consist of a short, flat acrosomal cap and, with the exception of the Carabus species, show long nuclei which extend parallel to the axonemes, along the flagella. Two relatively small mitochondrial derivatives and two small accessory bodies flank the axoneme. These bodies become very thick in the posterior flagellar region of D. atricapillus. The study revealed different spermatozeugma models and different sperm organization in the Carabidae family.
研究了来自不同步甲科部落的七个物种的精子结构。 Carabus preslii 和 Carabus granulatus interstitialis 形成精子束（精子栉），其中常规昆虫精子细胞的头部嵌入顶端帽（精子栉）中，留下后鞭毛自由。相反， Pterostichus morio 、 Pterostichus melas 、 Pterostichus melanarius 和 Amara aulica 形成复杂的精子栉，带有明显的轴向精子栉，与精子的侧群相关联。单个精子细胞包含在由精子栉的皮质区域的层状延伸限定的腔室中。 Demetrias atricapillus 与上述物种具有共同的一般精子栉结构，但前精子栉区域具有不同的结构，并且在后部，精子的侧群与精子栉分离，但通过由精子栉的皮质区域的延伸组成的柄与它保持连接。在研究中检查的物种的精子由短而平的顶体帽组成，除了 Carabus 物种外，还显示出长的核，这些核与轴突平行延伸，沿着鞭毛延伸。两个相对较小的线粒体衍生物和两个小的附属体位于轴突周围。这些体在 D. atricapillus 的后鞭毛区域变得非常厚。该研究揭示了步甲科家族中不同的精子栉模型和不同的精子组织。
