State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao 266237, China.
Department of Research and Development, Uttaranchal University, Dehradun 248007, India.
Cells. 2019 Jun 3;8(6):530. doi: 10.3390/cells8060530.
Two unrecognizable strains of the same bacterial species form a distinct colony boundary. During growth as colonies, uses multiple factors to establish cooperation between recognized strains and prevent interactions with unrecognized strains of the same species. Here, is a mutant strain deficient in immunity for the paired nuclease gene, , that has a function in the colony-merger incompatibility of DK1622. With the aim to investigate the factors involved in boundary formation, a proteome and metabolome study was employed. Visualization of the boundary between DK1622 and Δ was done scanning electron microscope (SEM), which displayed the presence of many damaged cells in the boundary. Proteome analysis of the DK1622- boundary disclosed many possible proteins, such as cold shock proteins, cell shape-determining protein MreC, along with a few pathways, such as RNA degradation, phenylalanine, tyrosine and tryptophan biosynthesis, and Type VI secretion system (T6SS), which may play major roles in the boundary formation. Metabolomics studies revealed various secondary metabolites that were significantly produced during boundary formation. Overall, the results concluded that multiple factors participated in the boundary formation in , leading to cellular damage that is helpful in solving the mystery of the boundary formation mechanism.
两种无法识别的同种细菌菌株形成明显的菌落边界。在作为菌落生长的过程中， 利用多种因素在被识别菌株之间建立合作关系，并防止与同种未被识别的菌株相互作用。在这里， 是一种缺乏配对核酸酶基因 的免疫缺陷突变体， 在 DK1622 的菌落融合不相容性中具有功能。为了研究边界形成涉及的因素，进行了蛋白质组学和代谢组学研究。通过扫描电子显微镜 (SEM) 对 DK1622 和 Δ 之间的边界进行可视化，显示边界处存在许多受损细胞。对 DK1622-边界的蛋白质组学分析揭示了许多可能的蛋白质，如冷休克蛋白、细胞形状决定蛋白 MreC，以及一些途径，如 RNA 降解、苯丙氨酸、酪氨酸和色氨酸生物合成以及 VI 型分泌系统 (T6SS)，它们可能在边界形成中起主要作用。代谢组学研究表明，在边界形成过程中会产生各种显著的次生代谢物。总的来说，结果表明，多种因素参与了 的边界形成，导致细胞损伤，有助于解决边界形成机制的奥秘。
