Animal Genomics Lab, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, India.
Amity Institute of Biotechnology, Amity University, Raipur, India.
Anim Biotechnol. 2020 Oct;31(5):432-439. doi: 10.1080/10495398.2019.1615497. Epub 2019 Jun 4.
The heat shock factors are important as they are master regulator of heat shock response. There are only few mammalian which have been characterized, namely , , and . The present study was aimed to clone and sequence characterize the partial open reading frames (ORFs) of and gene from cDNA isolated from testicular tissue of sheep (Macheri) and goat (Beetal). The partial ORFs of gene was observed to be 1627 bp in sheep and 1179 bp in goat and for it is 1137 bp in sheep and 1027 bp in goat. and encode a putative protein of 593 and 461 amino acid in goat and 568 and 553 amino acid in sheep, respectively. Phylogenetic analysis between the different orthologs suggested that these proteins are conserved from bovine to humans as well as in other mammals. Further, domain analyses using PredictNLS, MARCOIL and NetNES revealed that the members of protein orthologs contained all major domains, i.e., DNA-binding domain (DBD) and oligomerization domain (HR-A/B, and HR-C). The 3D structure of sheep and goat protein was predicted using SWISS-MODEL, which showed similar confirmation with the human protein sequence showing functional similarity between them.
热休克因子是重要的，因为它们是热休克反应的主要调节因子。目前已经鉴定出的哺乳动物热休克因子只有少数几种，即、、和。本研究旨在从绵羊（马奇里）和山羊（贝特尔）睾丸组织中分离的 cDNA 中克隆和测序特征分析和基因的部分开放阅读框（ORF）。在绵羊中观察到基因的部分 ORF 为 1627bp，在山羊中为 1179bp，而在绵羊中为 1137bp，在山羊中为 1027bp。和分别编码山羊 593 和 461 个氨基酸的假定蛋白，绵羊 568 和 553 个氨基酸的假定蛋白。不同直系同源物之间的系统发育分析表明，这些蛋白质在牛与人以及其他哺乳动物中都是保守的。此外，使用 PredictNLS、MARCOIL 和 NetNES 进行的结构域分析表明，和蛋白的同源物都包含所有主要结构域，即 DNA 结合结构域（DBD）和寡聚化结构域（HR-A/B 和 HR-C）。利用 SWISS-MODEL 预测了绵羊和山羊的蛋白 3D 结构，与人类的蛋白序列具有相似的构象，表明它们之间具有功能相似性。
