Molecular characterization and protein structure prediction of heat shock transcriptional factors in goat () and sheep ().

The heat shock factors are important as they are master regulator of heat shock response. There are only few mammalian which have been characterized, namely , , and . The present study was aimed to clone and sequence characterize the partial open reading frames (ORFs) of and gene from cDNA isolated from testicular tissue of sheep (Macheri) and goat (Beetal). The partial ORFs of gene was observed to be 1627 bp in sheep and 1179 bp in goat and for it is 1137 bp in sheep and 1027 bp in goat. and encode a putative protein of 593 and 461 amino acid in goat and 568 and 553 amino acid in sheep, respectively. Phylogenetic analysis between the different orthologs suggested that these proteins are conserved from bovine to humans as well as in other mammals. Further, domain analyses using PredictNLS, MARCOIL and NetNES revealed that the members of protein orthologs contained all major domains, i.e., DNA-binding domain (DBD) and oligomerization domain (HR-A/B, and HR-C). The 3D structure of sheep and goat protein was predicted using SWISS-MODEL, which showed similar confirmation with the human protein sequence showing functional similarity between them.