Marvel J, Tassignon J, Brait M, Meek K, Milner E C, Moser M, Capra J D, Urbain J
Laboratoire de Physiologie Animale, Université Libre de Bruxelles, Faculté des Sciences, Belgium.
Mol Immunol. 1987 May;24(5):463-9. doi: 10.1016/0161-5890(87)90020-4.
It has been previously shown that it is possible to modify the expressed repertoire of a given individual using idiotypic manipulation. For example, A/J mice respond to arsonate challenge by synthesizing a dominant idiotype, CRIA, whereas BALB/c mice do not. However, after treatment with rabbit polyclonal anti-CRIA antibodies (Ab2 or anti-idiotypic antibodies) and arsonate, BALB/c mice are able to synthesize a CRIA-like idiotype. To determine whether this modification of repertoire is dependent on the immunoglobulin loci (Ig-h, kappa), we have analyzed the anti-arsonate response after anti-idiotypic treatment of three strains of mice (C58, C.C58, AKR), chosen because they are among a small group of strains which express Kappa V regions not seen in other strains. There are also L chains lacking in these strains which are expressed in other mice. The C58 and C.C58 strains share the same Ig-h locus (Ig-ha) with BALB/c mice but C.C58 are congenic mice, that express the kappa loci on a BALB/c genetic background. AKR mice express the Ig-hd haplotype. AKR, C58 and C.C58 do not produce CRIA positive antibodies in response to arsonate; a defect which has been previously mapped to the kappa locus. These three strains of mice (C58, C.C58 and AKR) were treated with rabbit anti-CRIA and boosted with Ars-KLH. The results show that after such treatment, the C.C58 mice were able to express CRIA-like antibodies which are serologically identical to those of BALB/c.
先前已经表明，利用独特型操作改变给定个体的表达库是可能的。例如，A/J小鼠通过合成显性独特型CRIA来应对砷酸盐攻击，而BALB/c小鼠则不会。然而，在用兔多克隆抗CRIA抗体（Ab2或抗独特型抗体）和砷酸盐处理后，BALB/c小鼠能够合成一种类似CRIA的独特型。为了确定这种库的改变是否依赖于免疫球蛋白基因座（Ig-h、κ），我们分析了三种小鼠品系（C58、C.C58、AKR）在抗独特型处理后的抗砷酸盐反应，选择这三种品系是因为它们属于一小群表达其他品系中未见的κV区的品系。这些品系中还缺乏在其他小鼠中表达的轻链。C58和C.C58品系与BALB/c小鼠共享相同的Ig-h基因座（Ig-ha），但C.C58是同源基因小鼠，在BALB/c遗传背景上表达κ基因座。AKR小鼠表达Ig-hd单倍型。AKR、C58和C.C58对砷酸盐攻击不产生CRIA阳性抗体；这一缺陷先前已定位到κ基因座。用兔抗CRIA处理这三种小鼠品系（C58、C.C58和AKR），并用Ars-KLH加强免疫。结果表明，经过这样的处理后，C.C58小鼠能够表达与BALB/c小鼠血清学相同的类似CRIA的抗体。
