Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, P.O. Box 15719-14911, Tehran, Iran.
Department of Physiology and Pharmacology, Pasteur Institute of Iran, P.O. Box 1316943551, Tehran, Iran.
BMC Complement Altern Med. 2019 Jun 4;19(1):116. doi: 10.1186/s12906-019-2522-8.
Allium species are magnificently nutritious and are commonly used as a part of the diet in Iran. They have health enhancing benefits including anticancer properties due to the presence of numerous bioactive compounds. Herein, we investigated in vitro and in vivo anticancer properties of Allium bakhtiaricum extracts.
Anti-growth activity of different fractions was explored in vitro on different cancerous cells using MTT assay, Annexin V/PI and SA-β-gal staining, Western blotting, flowcytometric and immunofluorescence microscopic evaluations. In vivo antitumor activity was investigated in BALB/c mice bearing 4 T1 mammary carcinoma cells.
We demonstrated that chloroformic and ethyl acetate fractions exert cytotoxic activity toward MDA-MB-231 cells, the most sensitive cell line, after 72 h of treatment with IC values of 0.005 and 0.006 mg/ml, respectively. Incubation of MDA-MB-231 cells with ¼ and ½ IC concentrations of each fraction resulted in a significant G2/M cell cycle arrest. ¼ IC concentration of the chloroform fraction led to the disruption of polymerization in mitotic microtubules. Exposure of human breast cancer cells to different concentrations of the extracts at different incubation times did not induce apoptosis, autophagy or senescence. Our in vivo study revealed that administration of the chloroform extract at a dose of 1 mg/kg/day strongly suppressed mammary tumor progression and decreased the number of proliferative cells in the lung tissues indicating its anti-metastatic effect.
Our findings imply that the chloroform fraction of Allium bakhtiaricum possesses the suppressive action on breast cancer through mitotic cell cycle arrest suggesting a mechanism associated with disturbing microtubule polymerization.
葱属植物营养丰富，在伊朗常作为饮食的一部分。由于含有许多生物活性化合物，它们具有增强健康的益处，包括抗癌特性。在此，我们研究了 Allium bakhtiaricum 提取物的体外和体内抗癌特性。
使用 MTT 测定法、Annexin V/PI 和 SA-β-gal 染色、Western blot 分析、流式细胞术和免疫荧光显微镜评估，研究了不同部位在不同癌细胞中的抗生长活性。在荷 4T1 乳腺癌细胞的 BALB/c 小鼠中进行体内抗肿瘤活性研究。
我们证明氯仿和乙酸乙酯部位对 MDA-MB-231 细胞具有细胞毒性活性，最敏感的细胞系在处理 72 小时后，IC 值分别为 0.005 和 0.006mg/ml。MDA-MB-231 细胞与各部位的 1/4 和 1/2 IC 浓度孵育导致 G2/M 细胞周期阻滞。氯仿部位的 1/4 IC 浓度导致有丝分裂微管的聚合破坏。不同浓度的提取物在不同孵育时间暴露于人乳腺癌细胞不会诱导细胞凋亡、自噬或衰老。我们的体内研究表明，以 1mg/kg/天的剂量给予氯仿提取物强烈抑制乳腺肿瘤的进展，并减少肺组织中增殖细胞的数量，表明其具有抗转移作用。
我们的研究结果表明，Allium bakhtiaricum 的氯仿部位通过有丝分裂细胞周期阻滞对乳腺癌具有抑制作用，提示与干扰微管聚合相关的机制。
