Rodríguez-García Álvaro, Mares Rosa E, Muñoz Patricia L A, Meléndez-López Samuel G, Licea-Navarro Alexei F, Ramos Marco A
Facultad de Ciencias Químicas e Ingeniería, Universidad Autónoma de Baja California, Calzada Universidad 14418, Tijuana, BCN 22390, Mexico.
Laboratorio de Diagnóstico Clínico, Hospital General de Tijuana, Vía de la Juventud Oriente 4910, Tijuana, BCN 22010, Mexico.
Methods Protoc. 2018 Oct 23;1(4):37. doi: 10.3390/mps1040037.
Tuberculosis (TB) remains as a major public health issue in developing countries. Accurate detection is essential for the proper management of patients with active disease. Here, we present a simple DNAzol-LAMP (loop-mediated isothermal amplification) procedure for the detection of in sputum specimens. Twenty smear-positive sputum samples were analyzed as follows: (i) Genetic material was extracted by a standard DNAzol protocol, and (ii) mycobacterial DNA was detected by a typical TB-specific loop-mediated isothermal amplification method. Results and diagnostic test performance attests to the suitability of the proposed procedure.
结核病在发展中国家仍是一个主要的公共卫生问题。准确检测对于活动性疾病患者的妥善管理至关重要。在此，我们提出一种简单的DNAzol-环介导等温扩增（LAMP）程序用于检测痰标本中的[此处原文缺失相关检测对象]。对20份涂片阳性痰标本进行如下分析：（i）通过标准DNAzol方案提取遗传物质，以及（ii）采用典型的结核特异性环介导等温扩增方法检测分枝杆菌DNA。结果及诊断测试性能证明了所提出程序的适用性。
