Chen Weidong, Wang Jinzeng, Shi Jiahao, Yang Xu, Yang Ping, Wang Ning, Yang Sai, Xie Tianpei, Yang Hua, Zhang Mengjie, Wang Haiyun, Fei Jian
1School of Life Science and Technology, Tongji University, Shanghai 200092, China.
3School of Medicine, Tongji University, Shanghai 200092, China.
Aging Dis. 2019 Jun 1;10(3):578-591. doi: 10.14336/AD.2018.0604. eCollection 2019 Jun.
Liuwei Dihuang (LWDH), a famous traditional Chinese medicine, is widely used in the clinical treatment of aging-related diseases in China. However, its pharmacological mechanisms are not clear. In the present study, we evaluated the lifespan extension effect of LWDH in and mice and revealed its underlying mechanisms. The results showed that LWDH significantly extended the lifespan of in a dose-dependent manner. LWDH also conferred protection to nematodes against oxidative stress and reduced their fat storage. Genetics analysis and microarray data showed that the longevity effect of LWDH was attributed to the regulation of the innate immune response, proteolysis, lipid metabolism, and the oxidation-reduction process and was dependent on Among the six herbs in the formula, Radix Rehmanniae Preparata and Fructus Macrocarpii contributed most to the longevity effect of this medicine, while the other four components had a synergistic effect on the longevity effect of the prescription. The lack of any single herb reduced the efficacy of the complete formula. LWDH also extended the lifespan and reduced both the weight and oxidant stress status in aged mice. Taken together, these results suggested that LWDH might function in a multi-target manner to extend the lifespan in both and aged mice, and the best effect was achieved with the complete formula.
六味地黄丸（LWDH）是一种著名的中药，在中国被广泛用于与衰老相关疾病的临床治疗。然而，其药理机制尚不清楚。在本研究中，我们评估了六味地黄丸对秀丽隐杆线虫和小鼠的寿命延长作用，并揭示了其潜在机制。结果表明，六味地黄丸以剂量依赖的方式显著延长了秀丽隐杆线虫的寿命。六味地黄丸还赋予线虫抗氧化应激能力，并减少其脂肪储存。遗传学分析和微阵列数据表明，六味地黄丸的长寿作用归因于对先天免疫反应、蛋白水解、脂质代谢和氧化还原过程的调节，并且依赖于DAF-16。在该配方的六味药中，熟地黄和山楂对该药的长寿作用贡献最大，而其他四味药对该方剂的长寿作用具有协同作用。缺少任何一味药都会降低整个配方的疗效。六味地黄丸还延长了老年小鼠的寿命，并减轻了其体重和氧化应激状态。综上所述，这些结果表明，六味地黄丸可能以多靶点方式发挥作用，延长秀丽隐杆线虫和老年小鼠的寿命，完整配方的效果最佳。
