Wan Fengqi, Zhi Dejuan, Liu Dongling, Xian Jun, Li Menghui, AbuLizi Aimaier, Ju Weishuang, Li Hongyu
School of Pharmacy, Lanzhou University, No. 99 Donggang West Road, Chengguan District, Lanzhou, China.
Biogerontology. 2014 Aug;15(4):377-87. doi: 10.1007/s10522-014-9508-1. Epub 2014 Jun 22.
There is considerable interest in using traditional Chinese medicine formulas (TCMF) to delay aging or treat age-related diseases. Due to cost and duration, the beneficial effects of TCMF on prolongation are mainly extrapolated from vitro studies or physiological indexes. Little is known about whether TCMF are beneficial in whole level, particularly with respect to lifespan. To address this issue, we selected eight formulas with anti-oxidative activity and examined their effects on the lifespan of Caenorhabditis elegans. The results showed that seven of the eight formulas could prolong lifespan of TK22 mutant significantly and five of the eight formulas could obviously extend lifespan of N2 wild-type. To further characterize the prolongation effects, oxidative stress, thermal stress and reproduction test were assayed. We found that the formulas that extended lifespan of TK22 could also protect it from oxidative stress, without reducing the reproductive capacity. Meanwhile, the formulas that prolonged lifespan of N2 wild-type could also enhance its resistance against thermal stress, with damaging the reproductive fitness. These observations indicate that TCMF used in our experiment could be potential therapeutics for anti-aging.
人们对使用中药配方来延缓衰老或治疗与年龄相关的疾病有着浓厚兴趣。由于成本和时间问题，中药配方在延长寿命方面的有益效果主要是从体外研究或生理指标推断而来。关于中药配方在整体层面是否有益，尤其是对寿命的影响，人们所知甚少。为了解决这个问题，我们选择了八种具有抗氧化活性的配方，并研究了它们对秀丽隐杆线虫寿命的影响。结果表明，八种配方中的七种能够显著延长TK22突变体的寿命，八种配方中的五种能够明显延长N2野生型的寿命。为了进一步表征延长寿命的效果，我们进行了氧化应激、热应激和繁殖测试。我们发现，能够延长TK22寿命的配方也能保护其免受氧化应激，而不会降低繁殖能力。同时，能够延长N2野生型寿命的配方也能增强其对热应激的抵抗力，而不会损害生殖适应性。这些观察结果表明，我们实验中使用的中药配方可能是潜在的抗衰老疗法。
