DEAH 框 RNA 解旋酶 DHX38 与参与染色体分离的卫星 I 非编码 RNA 结合。

DEAH box RNA helicase DHX38 associates with satellite I noncoding RNA involved in chromosome segregation.

机构信息

Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto, Japan.

Department of Medical Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto, Japan.

出版信息

Genes Cells. 2019 Aug;24(8):585-590. doi: 10.1111/gtc.12707. Epub 2019 Jun 28.

DOI:10.1111/gtc.12707

PMID:31166646

Abstract

Noncoding (nc) RNA called satellite I is transcribed from the human centromere region. Depletion of this ncRNA results in abnormal nuclear morphology because of defects in chromosome segregation. Some protein factors interact with this ncRNA and function as a component of a nc ribonucleoprotein (RNP) complex in mitotic regulation. Here, we found that DHX38, a pre-mRNA splicing-related DEAH box RNA helicase, interacts with satellite I ncRNA. Depletion of DHX38 resulted in defective chromosome segregation similar to knockdown of satellite I ncRNA. Interaction between DHX38 and ncRNA was interphase-specific, but DHX38 depletion affected the function of Aurora B, which associated with satellite I ncRNA at mitotic phase. Based on these findings, we suggest that DHX38 has a role in mitotic regulation as a component of the satellite I ncRNP complex at interphase.

摘要

非编码（nc）RNA 称为卫星 I 从人类着丝粒区域转录。这种 ncRNA 的耗竭导致染色体分离缺陷，从而导致核形态异常。一些蛋白质因子与这种 ncRNA 相互作用，并作为有丝分裂调节中 nc 核糖核蛋白（RNP）复合物的一个组成部分发挥作用。在这里，我们发现，与前体 mRNA 剪接相关的 DEAH 盒 RNA 解旋酶 DHX38，与卫星 I ncRNA 相互作用。DHX38 的耗竭导致与卫星 I ncRNA 敲低相似的染色体分离缺陷。DHX38 与 ncRNA 之间的相互作用是间期特异性的，但 DHX38 的耗竭影响了与卫星 I ncRNA 在有丝分裂阶段相关的 Aurora B 的功能。基于这些发现，我们认为 DHX38 作为卫星 I ncRNP 复合物的一个组成部分，在有丝分裂调节中发挥作用。