Davies M, Sabbadini E
Cancer Res. 1979 Mar;39(3):959-65.
The study was undertaken of the ability of Bacillus Calmette-Guérin (BCG), used in combination with a live tumor cell vaccine, to potentiate systemic antitumor immunity in rats. BCG was administered in two injections, with the first one given i.v. and the second one given intradermally mixed with the tumor cell vaccine 12 days later at the time of implant of an s.c. tumor, which served to monitor antitumor immunity. Both these BCG injections were necessary to obtain maximal protection measured in terms of increased survival rates of the rats and of decreased tumor growth rates. The dose of BCG in both the first and second injections was critical for optimal protection, since low doses (125 to 250 micrograms) afforded protection, while higher doses (over 1500 micrograms) decreased or abolished the protective effect. These results strongly suggest that BCG, only if administered under the appropriate conditions, is able to potentiate systemic tumor immunity and to provide a significant level of protection for a tumor-bearing animal.
本研究旨在探讨卡介苗（BCG）与活肿瘤细胞疫苗联合使用时增强大鼠全身抗肿瘤免疫的能力。卡介苗分两次注射，第一次静脉注射，12天后第二次皮下注射肿瘤细胞疫苗时皮内注射，皮下肿瘤植入用于监测抗肿瘤免疫。这两次卡介苗注射对于以大鼠存活率提高和肿瘤生长速率降低来衡量的最大程度保护都是必要的。第一次和第二次注射的卡介苗剂量对于最佳保护至关重要，因为低剂量（125至250微克）能提供保护，而高剂量（超过1500微克）会降低或消除保护作用。这些结果强烈表明，卡介苗只有在适当条件下给药才能增强全身肿瘤免疫，并为荷瘤动物提供显著水平的保护。
