Laboratory of Soil Science, Faculty of Agriculture, Aristotle University of Thessaloniki, 541 24, Thessaloniki, Greece.
Laboratory of Plant Pathology, Faculty of Agriculture, Aristotle University of Thessaloniki, 541 24, Thessaloniki, Greece.
Arch Microbiol. 2019 Nov;201(9):1151-1161. doi: 10.1007/s00203-019-01679-z. Epub 2019 Jun 5.
The Prespa lakes plain is an isolated area where about 1000 ha are seeded to Phaseolus vulgaris L. and Phaseolus coccineus L. Nodulation, arbuscular mycorrhizal fungal (AMF) presence and the genetic diversity of rhizobia were evaluated by 16S-ITS-23S-RFLP patterns and by sequencing. The bean rhizobial population in the region was diverse, despite its geographic isolation. No biogeographic relationships were detected, apart from a Rhizobium tropici-related strain that originated from an acidic soil. No clear pattern was detected in clustering with bean species and all isolates formed nodules with both bean species. Most strains were related to Rhizobium leguminosarum and a number of isolates were falling outside the already characterized species of genus Rhizobium. Application of heavy fertilization has resulted in high soil N and P levels, which most likely reduced nodulation and AMF spore presence. However, considerable AMF root length colonization was found in most of the fields.
普雷斯帕湖平原是一个孤立的地区，约有 1000 公顷的土地种植了普通菜豆和多花菜豆。通过 16S-ITS-23S-RFLP 模式和测序评估了根瘤菌的结瘤、丛枝菌根真菌（AMF）存在和遗传多样性。尽管该地区地理位置孤立，但豆科根瘤菌种群具有多样性。除了一种起源于酸性土壤的与 Rhizobium tropici 相关的菌株外，没有检测到生物地理关系。与豆科植物的聚类没有明显的模式，所有分离株都与两种豆科植物形成根瘤。大多数菌株与根瘤菌属的 Rhizobium leguminosarum 有关，一些分离株属于已鉴定的根瘤菌属种之外。大量施肥导致土壤氮和磷水平升高，这很可能减少了结瘤和 AMF 孢子的存在。然而，在大多数田地中发现了相当数量的 AMF 根长定殖。
