Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, P. R. China.
Suzhou Municipal Center for Disease Prevention and Control, Suzhou, P. R. China.
J Sep Sci. 2019 Aug;42(16):2696-2705. doi: 10.1002/jssc.201900224. Epub 2019 Jun 19.
We have developed and validated a simple, eco-friendly, and reliable method to simultaneously determine paracetamol and chloramphenicol in meat with ultra performance liquid chromatography and tandem mass spectrometry. The samples were firstly extracted with ethylenediaminetetraacetic acid-McIlvaine's buffer and then purified by solid-phase extraction by using a novel core-shell polyaniline/polyacrylonitrile nanofibers mat. Compared with existing methods for the two analytes, the proposed method was simplified greatly with much fewer sample preparation steps, consumed much less time (< 2 min per sample) and organic solvent (0.7 mL per sample). Low detection levels (0.15-0.20 µg/kg for paracetamol, 0.01 µg/kg for chloramphenicol) with good precision and recoveries of the target compounds were obtained. The proposed method was applied to determine the residual paracetamol and chloramphenicol in pork, chicken, and beef samples, and the test results were consistent with those using the Chinese national standard methods, which demonstrates the reliability and practicality of the new method.
我们开发并验证了一种简单、环保、可靠的超高效液相色谱-串联质谱法，可同时测定肉中扑热息痛和氯霉素的含量。样品首先用乙二胺四乙酸-柠檬酸缓冲液提取，然后用新型核壳聚苯胺/聚丙烯腈纳米纤维垫通过固相萃取进行纯化。与现有的两种分析物的方法相比，该方法大大简化，样品制备步骤少得多，耗时（<2 分钟/样品）和有机溶剂（<0.7 毫升/样品）用量少。该方法的检测限低（扑热息痛为 0.15-0.20μg/kg，氯霉素为 0.01μg/kg），具有良好的精密度和目标化合物的回收率。该方法应用于测定猪肉、鸡肉和牛肉样品中残留的扑热息痛和氯霉素，测试结果与中国国家标准方法一致，证明了新方法的可靠性和实用性。
