Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Science Centre, Manchester, UK.
Evotec UK Ltd, Block 23 Alderley Park, Macclesfield SK10 4TG, UK.
J Med Microbiol. 2019 Jul;68(7):1047-1052. doi: 10.1099/jmm.0.001007. Epub 2019 Jun 6.
Miramistin is a topical antiseptic with broad antimicrobial activity that was developed in the Soviet Union during the Cold War.
To investigate the antifungal activity of miramistin against clinically relevant drug-resistant fungi.
The in vitro activity of miramistin was determined following Clinical and Laboratory Standards Institute (CLSI) guidelines. Mammalian cell toxicity was tested using a McCoy cell line and topical and systemic tolerability, and in vivo efficacy was tested using Galleria mellonella models.
The minimal inhibitory concentration (MIC) range against fungi was 1.56-25 mg l (GM 3.13 mg l ). In the G. mellonella model, miramistin provided potent survival benefits for Candida albicans and Aspergillus fumigatus infection. Miramistin was tolerated by McCoy cell lines at concentrations up to 1000 mg l and was systemically safe in G. mellonella at 2000 mg kg. Topical administration at 32 000 mg l was well tolerated with no adverse effects.
These findings support further investigation of miramistin and suggest its possible use for treatment of superficial fungal infections.
米利金是一种具有广泛抗菌活性的局部抗菌剂，由苏联在冷战期间开发。
研究米利金对临床相关耐药真菌的抗真菌活性。
根据临床和实验室标准协会 (CLSI) 指南测定米利金的体外活性。使用 McCoy 细胞系测试哺乳动物细胞毒性，使用大蜡螟模型测试局部和全身耐受性以及体内功效。
针对真菌的最小抑菌浓度 (MIC) 范围为 1.56-25 毫克/升（GM 为 3.13 毫克/升）。在大蜡螟模型中，米利金为白色念珠菌和烟曲霉感染提供了有效的生存益处。米利金在高达 1000 毫克/升的浓度下被 McCoy 细胞系耐受，在大蜡螟中以 2000 毫克/千克的剂量全身使用安全。32000 毫克/升的局部给药耐受性良好，无不良反应。
这些发现支持对米利金的进一步研究，并表明其可能用于治疗浅表真菌感染。
