Haas D D, Ware B R
Biochemistry. 1978 Nov 14;17(23):4946-50. doi: 10.1021/bi00616a014.
Diffusion studies by photon correlation of scattered laser light confirm the dissociation of the tetrameric form of human carboxyhemoglobin to dimers above pH 10 and provide new estimates of the subunit dissociation equilibrium constants in this pH range. Electrophoretic light-scattering experiments under the same conditions reveal that the electrophoretic mobilities of tetramers and dimers are indistinguishable to within instrumental resolution (ca. 7% in these experiments). The data imply an increase of the electrical charge on the dimer of at least 2.8 to 4.4 net negative charges upon dissociation. Mechanisms for the accumulation of negative charge by the dimer upon dissociation of the tetramer are proposed.
通过对散射激光光进行光子相关分析的扩散研究证实，在pH值高于10时，人羧基血红蛋白的四聚体形式会解离为二聚体，并提供了该pH范围内亚基解离平衡常数的新估计值。在相同条件下进行的电泳光散射实验表明，四聚体和二聚体的电泳迁移率在仪器分辨率范围内无法区分（在这些实验中约为7%）。数据表明，解离时二聚体上的电荷增加了至少2.8至4.4个净负电荷。文中提出了四聚体解离时二聚体积累负电荷的机制。
