Lunelli L, Zuliani P, Baldini G
Dipartimento di Fiscia, Università degli Studi, Milano, Italy.
Biopolymers. 1994 Jun;34(6):747-57. doi: 10.1002/bip.360340607.
Bovine carbonmonoxy hemoglobin investigated with light scattering studies is found to dissociate from its native tetramer structure into dimers and monomers. The values of the hydrated tetramer radius, RT = 32.1 A, and the fractional dissociation vs pH, have been obtained at different ionic strengths from the autocorrelation function of the scattered light. The results suggest that a relevant contribution to Hb dissociation is due to electrostatic effects and, by means of a model derived by Tanford, it has been possible to predict the behavior of dissociation. Among the findings of this approach, we recall the estimates of the electrostatic energy contributions to Hb dissociation, up to congruent to 6RT, and the predicted charge of tetrameric Hb vs pH, which agrees very well with the experimental data.
通过光散射研究对牛碳氧血红蛋白进行研究后发现，它会从其天然的四聚体结构解离成二聚体和单体。在不同离子强度下，从散射光的自相关函数中获得了水合四聚体半径(R_T = 32.1 Å)的值以及解离分数与pH的关系。结果表明，对血红蛋白解离有重要贡献的是静电效应，并且借助Tanford推导的模型，已经能够预测解离行为。在这种方法的研究结果中，我们回顾了对血红蛋白解离的静电能量贡献的估计，高达约(6R_T)，以及预测的四聚体血红蛋白电荷与pH的关系，这与实验数据非常吻合。
