Laboratory of Cell Function and Structure, Graduate School of Agricultural Science, Kobe University, Nada Kobe, 657-8501, Japan.
Agrivirology Laboratory, Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama, 710-0046, Japan.
Virology. 2019 Aug;534:25-35. doi: 10.1016/j.virol.2019.05.015. Epub 2019 May 25.
Three ourmia-like viruses, designated Pyricularia oryzae ourmia-like virus (PoOLV) 1 to 3, were identified in a wheat-infecting isolate of P. oryzae. The sizes of the full-length PoOLV1-3 genomes were determined to be 2,528, 1,671, and 2,557 nt. Interestingly, we also found two abundant single-stranded RNAs sharing their 5' terminal 25 and 255 nt with PoOLV1 RNA and PoOLV3 RNA, respectively. The PoOLV1- and PoOLV3-associated RNAs (ARNA1 and ARNA3) were 639 and 514 nt in length, and possessed one and two small ORFs, respectively. In the field isolates of P. oryzae, PoOLVs and ARNAs were detectable at varying levels, and the levels of PoOLV1 and ARNA1 as well as those of PoOLV3 and ARNA3, were tightly correlated. In addition, gene silencing of PoOLV1 and PoOLV3 resulted in a reduction of ARNA1 and ARNA3, respectively. There results indicated that replication of ARNA1 and ARNA3 was associated with PoOLV1 and PoOLV3, respectively.
三种类似于稻瘟病菌的病毒，分别命名为稻瘟病菌ourmia 样病毒（PoOLV）1 至 3，在感染小麦的稻瘟病菌分离株中被鉴定出来。全长 PoOLV1-3 基因组的大小分别为 2528、1671 和 2557nt。有趣的是，我们还发现了两种丰富的单链 RNA，它们的 5' 端分别与 PoOLV1 RNA 和 PoOLV3 RNA 共享 25 和 255nt。PoOLV1 和 PoOLV3 相关的 RNA（ARNA1 和 ARNA3）分别长 639 和 514nt，分别具有一个和两个小 ORF。在稻瘟病菌田间分离物中，PoOLVs 和 ARNAs 的检测水平不同，PoOLV1 和 ARNA1 的水平以及 PoOLV3 和 ARNA3 的水平紧密相关。此外，PoOLV1 和 PoOLV3 的基因沉默导致 ARNA1 和 ARNA3 分别减少。这些结果表明，ARNA1 和 ARNA3 的复制分别与 PoOLV1 和 PoOLV3 相关。
