Hubei key Laboratory of Biological Resources Protection and Utilization, College of Forestry and Horticulture, Hubei Minzu University, Enshi, 445000, People's Republic of China.
State Key Laboratory of Agricultural Microbiology, The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Arch Virol. 2020 Apr;165(4):1027-1031. doi: 10.1007/s00705-020-04556-z. Epub 2020 Feb 13.
Hypoviruses are positive-sense single-stranded RNA mycovirus that infect filamentous fungi. However, hypoviruses have not been reported in Bipolaris oryzae, an important phytopathogenic fungus in water bamboo and rice. Here, we report the characterization of a novel hypovirus, tentatively named "Bipolaris oryzae hypovirus 1" (BoHV1), isolated from strain ES35 of B. oryzae infecting water bamboo. The complete genome of BoHV1 consists of 13,596 nucleotides and a poly(A) tail at the 3' end. BoHV1 has single open reading frame (ORF) and encodes a putative polyprotein (4,218 amino acids) containing four potential conserved domains for a papain-like protease, a protein of unknown function (DUF3525), RNA-dependent RNA polymerase (RdRp), and helicase. Phylogenetic analysis of the polyprotein, RdRp, and helicase domains suggested that BoHV1 belongs to the genus Hypovirus within the family Hypoviridae. This is the first report of the presence of a hypovirus in the phytopathogenic fungus B. oryzae.
类病毒是感染丝状真菌的正链单链 RNA 真菌病毒。然而，在水竹和水稻上的一种重要植物病原菌——稻丝核菌中，尚未报道过类病毒的存在。在这里，我们报告了一种新型类病毒的特征，暂命名为“稻丝核菌类病毒 1”（BoHV1），它是从感染水竹的稻丝核菌 ES35 菌株中分离出来的。BoHV1 的完整基因组由 13596 个核苷酸和 3'端的 poly(A)尾巴组成。BoHV1 具有单个开放阅读框（ORF），并编码一个假定的多蛋白（4218 个氨基酸），其中包含四个潜在的保守结构域，用于木瓜蛋白酶样蛋白酶、功能未知蛋白（DUF3525）、RNA 依赖性 RNA 聚合酶（RdRp）和解旋酶。多蛋白、RdRp 和解旋酶结构域的系统发育分析表明，BoHV1 属于 Hypoviridae 科中的 Hypovirus 属。这是首次在植物病原菌稻丝核菌中发现类病毒。
