Department of Respiratory and Critical Care Medicine, The Second Medical College of Jinan University (Shenzhen People's Hospital), Shenzhen, China.
Shenzhen Key Laboratory of Respiratory Disease, Shenzhen, China.
Mol Genet Genomic Med. 2019 Jul;7(7):e00792. doi: 10.1002/mgg3.792. Epub 2019 Jun 7.
Tuberculosis (TB) is a major global health problem and has replaced HIV as the leading cause of death from a single infectious agent.
Here, we applied high throughput sequencing to study the immune repertoire of nine pulmonary tuberculosis patients and nine healthy control samples.
Tuberculosis patients and healthy controls displayed significantly different high express clones and distinguishable sharing of CDR3 sequences. The TRBV and TRBJ gene usage showed higher expression clones in patients than in controls and we also found specific high express TRBV and TRBJ gene clones in different groups. In addition, six highly expressed TRBV/TRBJ combinations were detected in the CD4 group, 21 in the CD8 group and 32 in the tissue group.
In conclusion, we studied the patients with tuberculosis as well as healthy control individuals in order to understand the characteristics of immune repertoire. Sharing of CDR3 sequences and differential expression of genes was found among the patients with tuberculosis which could be used for the development of potential vaccine and targets treatment.
结核病（TB）是一个全球性的主要健康问题，已取代艾滋病毒成为单一传染病原体导致死亡的主要原因。
在这里，我们应用高通量测序来研究九例肺结核患者和九例健康对照样本的免疫受体库。
肺结核患者和健康对照组显示出明显不同的高表达克隆和可区分的 CDR3 序列共享。TRBV 和 TRBJ 基因的使用显示患者组的高表达克隆高于对照组，我们还发现了不同组中特定的高表达 TRBV 和 TRBJ 基因克隆。此外，在 CD4 组中检测到六个高表达的 TRBV/TRBJ 组合，在 CD8 组中检测到 21 个，在组织组中检测到 32 个。
总之，我们研究了肺结核患者和健康对照个体，以了解免疫受体库的特征。在肺结核患者中发现了 CDR3 序列的共享和基因的差异表达，这可能用于开发潜在的疫苗和治疗靶点。
