Pathology Department, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Haematopathology Unit, Pathology Department, Hospital Clinic, University of Barcelona, Barcelona, Spain.
Histopathology. 2019 Nov;75(5):704-714. doi: 10.1111/his.13935. Epub 2019 Aug 19.
Mantle cell lymphoma (MCL) is a heterogeneous disease with an aggressive behaviour in most cases, which is associated with expression of sex determining region-Y-box11 (SOX11). Experimental studies have shown that SOX11 expression is associated with an angiogenic switch characterised by increased expression of angiogenic-related signatures and vascularisation of murine tumours. However, the relationship between angiogenesis and SOX11 expression in primary tumours is not well understood. Therefore, the aim of this study was to evaluate the development of microvascular angiogenesis in primary MCL in relation to SOX11 expression and its potential prognostic value.
Fifty-six patients diagnosed with MCL, 38 SOX11-positive and 18 SOX11-negative, were studied. The relative intratumoral microvascular area (MVA) and microvessel density (MVD) (number of intratumoral microvessels/μm ) were measured on CD34-stained slides using a computerised image analysis system. SOX11-positive MCL showed a significant higher microvascular development than negative tumours (median MVA = 14.5 × 10 versus 5.0 × 10 P < 0.001; median MVD = 18.6/μm versus 14.2/μm , P = 0.021). Analysing the MVA and MVD as continuous variables, a high MVD was associated with shorter overall survival (P = 0.004), and a similar tendency was observed for high MVA (P = 0.064). The microvascular development was not related to the Ki-67 proliferative index or 17p/TP53, 9p or 11q alterations.
These findings suggest that SOX11 promotes an angiogenic phenotype in primary MCL, which may contribute to the more aggressive behaviour of these tumours.
套细胞淋巴瘤（MCL）是一种具有侵袭性的异质性疾病，大多数情况下表达性别决定区 Y 框 11（SOX11）。实验研究表明，SOX11 表达与血管生成开关有关，其特征是血管生成相关特征的表达增加和鼠类肿瘤的血管化。然而，原发性肿瘤中血管生成与 SOX11 表达之间的关系尚不清楚。因此，本研究旨在评估原发性 MCL 中微血管血管生成的发展与 SOX11 表达的关系及其潜在的预后价值。
研究了 56 例诊断为 MCL 的患者，其中 38 例 SOX11 阳性，18 例 SOX11 阴性。使用计算机图像分析系统对 CD34 染色切片上的相对肿瘤内微血管面积（MVA）和微血管密度（MVD）（肿瘤内微血管数/μm）进行了测量。SOX11 阳性的 MCL 比阴性肿瘤的微血管发育明显更高（中位数 MVA=14.5×10 对 5.0×10，P<0.001；中位数 MVD=18.6/μm 对 14.2/μm，P=0.021）。分析 MVA 和 MVD 作为连续变量时，高 MVD 与总生存期较短相关（P=0.004），高 MVA 也有类似的趋势（P=0.064）。微血管发育与 Ki-67 增殖指数或 17p/TP53、9p 或 11q 改变无关。
这些发现表明，SOX11 促进原发性 MCL 中的血管生成表型，这可能有助于这些肿瘤更具侵袭性的行为。
