Stanford University, Stanford, CA, United States.
J Chem Neuroanat. 2019 Oct;100:101653. doi: 10.1016/j.jchemneu.2019.101653. Epub 2019 Jun 4.
Mapping brain connectivity at single cell resolution is critical for understanding brain structure. For decades, such mapping has been principally approached with microscopy techniques, aiming to visualize neurons and their connections. However, these techniques are often very labor intensive and do not scale well to the complexity of mammalian brains. We recently leveraged the speed and parallelization of DNA sequencing to map the projections of thousands of single neurons in single experiments, and to map cortical mesoscale connectivity in single mice. Here, I review the state of sequencing-based neuroanatomy, and discuss future directions in synaptic connectivity mapping and comparative connectomics.
以单细胞分辨率绘制大脑连接图谱对于理解大脑结构至关重要。数十年来，此类图谱绘制主要采用显微镜技术，旨在可视化神经元及其连接。然而，这些技术通常非常耗费人力，并且无法很好地扩展到哺乳动物大脑的复杂性。我们最近利用 DNA 测序的速度和并行性，在单次实验中绘制了数千个单个神经元的投射，并在单个小鼠中绘制了皮质介观连接图谱。在这里，我回顾了基于测序的神经解剖学的现状，并讨论了突触连接图谱绘制和比较连接组学的未来方向。
