Yan Fanyong, Sun Xiaodong, Zhang Ruiqi, Jiang Yingxia, Xu Jinxia, Wei Junfu
State Key Laboratory of Separation Membranes and Membrane Processes, National Center for International Joint Research on Separation Membranes, School of Chemistry and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300387, PR China.
State Key Laboratory of Separation Membranes and Membrane Processes, National Center for International Joint Research on Separation Membranes, School of Chemistry and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300387, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Nov 5;222:117222. doi: 10.1016/j.saa.2019.117222. Epub 2019 May 29.
Three enhanced fluorescence probes based on Rhodamine B-Schiff base structure were synthesized for detecting Cu. The corresponding detection limits were found to be 0.25 μM, 0.15 μM and 0.18 μM. Binding ratio and binding sites were determined by Job's and nuclear magnetic titration experiments. The binding constants obtained by the Benesi-Hildebrand equation to be 341.0 M,1.8 × 10 M, and 265.4 M, respectively. As isomers, the different effects of probes on Cu detection were researched. By adjusting the position and the size of the substituent group, the effects of binding sites and steric hindrance on the complexation ratio, response time and detection limit were discussed. Optimal spatial combination structure with Cu was obtained through energy calculation. Detection mechanism of Rhodamine B ring opening based on the complex of the Schiff base with Cu was confirmed. E. coli staining and detection of real water samples had expanded their applications.
合成了三种基于罗丹明B-席夫碱结构的增强型荧光探针用于检测铜。发现相应的检测限分别为0.25μM、0.15μM和0.18μM。通过Job法和核磁共振滴定实验确定了结合比和结合位点。根据Benesi-Hildebrand方程得到的结合常数分别为341.0 M、1.8×10 M和265.4 M。作为异构体，研究了探针在铜检测方面的不同效果。通过调整取代基的位置和大小，讨论了结合位点和空间位阻对络合比、响应时间和检测限的影响。通过能量计算获得了与铜的最佳空间组合结构。证实了基于席夫碱与铜的配合物的罗丹明B开环检测机制。大肠杆菌染色和实际水样检测拓展了它们的应用。
